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  本文選題：牛磺酸 + 錳�。� 參考：《廣西醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的：探討�；撬釋θ惧i大鼠丘腦氨基酸類神經(jīng)遞質(zhì)及其相關(guān)酶改變的干預(yù)作用。 方法：按隨機方法將SD大鼠分為空白對照組、染錳組、預(yù)防組、治療組和治療對照組,共5組。染錳組、預(yù)防組、治療組和治療對照組大鼠腹腔注射MnCl2-4H2O20mg/kg·d,其中預(yù)防組大鼠同時腹腔注射�；撬�200mg/kg·d,空白對照組腹腔注射等容量生理鹽水,連續(xù)8周,每周5天,染錳組、預(yù)防組和空白對照組試驗終止。然后,治療組大鼠繼續(xù)腹腔注射�；撬�200mg/kg·d,治療對照組大鼠腹腔注射等容量生理鹽水,連續(xù)8周。最后一次注射后24h進(jìn)行水迷宮實驗,分別以前5天平均逃避潛伏期及低6天的平臺搜索次數(shù)評估大鼠空間學(xué)習(xí)能力及空間記憶能力的變化；然后處死大鼠,切取丘腦。用BCA蛋白濃度測定試劑盒測定樣品中的蛋白含量,用高效液相色譜(]IPLC)紫外檢測法測定大鼠腦丘腦谷氨酸(glutamic acid, Glu)、谷氨酰胺(glutamine, Gln)和Y-氨基丁酸(gamma-aminobutyric acid, GABA)的含量；用酶聯(lián)免疫法(ELISA)測定谷氨酸脫羧酶(GAD)、磷酸激活谷氨酰胺酶(PAG)和谷氨酰胺合成酶(GS)的含量。 結(jié)果：1、染錳組大鼠逃避潛伏期時間較空白對照組明顯延長(P0.05),搜索到目標(biāo)區(qū)域次數(shù)明顯減少,差異有統(tǒng)計學(xué)意義(P0.05)。預(yù)防組大鼠逃避潛伏時間較染錳組明顯減少(P0.05),預(yù)防組大鼠搜索目標(biāo)區(qū)域次數(shù)與染錳組比較其差異有統(tǒng)計學(xué)意義(P0.05)。治療組大鼠逃避潛伏期較治療對照組明顯縮短(P0.05),治療組大鼠搜索目標(biāo)區(qū)域次數(shù)與治療對照組比較其差異有統(tǒng)計學(xué)意義(P0.05)。治療組大鼠逃避潛伏時間較染錳組明顯減少(P0.05),與空白對照組比較其差異有統(tǒng)計學(xué)意義(P0.05)；治療組大鼠搜索目標(biāo)區(qū)域次數(shù)與染錳組比較其差異有統(tǒng)計學(xué)意義(P0.05)2、染錳8周后,染錳組Glu含量比空白對照組含量高,差別有統(tǒng)計學(xué)意義(P0.05)；染錳組G1n和GABA含量比空白對照組低,其差異有統(tǒng)計學(xué)意義(P0.05)；染錳組PAG含量比空白對照組含量高,差異有統(tǒng)計學(xué)意義(P0.05),染錳組GAD和GS含量比空白對照組低,差異有統(tǒng)計學(xué)意義(P0.05)；�；撬犷A(yù)防8周時,預(yù)防組Glu含量比染錳組含量低,差異有統(tǒng)計學(xué)意義(P0.05)；預(yù)防組G1n和GABA含量比染錳組高,其差異有統(tǒng)計學(xué)意義(P0.05)；預(yù)防組PAG含量比染錳組含量低,差異有統(tǒng)計學(xué)意義(P0.05),預(yù)防組GAD和GS含量比染錳組高,差異有統(tǒng)計學(xué)意義(P0.05)。�；撬嶂委�8周后,治療組Glu含量均比治療對照組含量低,治療組G1n和GABA含量均比治療對照組含量高,差異有統(tǒng)計學(xué)意義(P0.05)；治療組PAG含量比治療對照組含量低,治療組GAD和GS含量均比治療對照組含量高,差異有統(tǒng)計學(xué)意義(P0.05)。治療組Glu含量比染錳組含量低,差異有統(tǒng)計學(xué)意義(P0.05)；治療組G1n和GABA含量比染錳組高,其差異有統(tǒng)計學(xué)意義(P0.05);治療組PAG含量比染錳組含量低,差異有統(tǒng)計學(xué)意義(P0.05),治療組GAD和GS含量比染錳組高,差異有統(tǒng)計學(xué)意義(P0.05)。 結(jié)論：錳可致大鼠丘腦Glu升高,Gln和GABA減低,�；撬釋﹀i所致大鼠丘腦Glu、Gln和GABA的改變有干預(yù)作用。
[Abstract]:Objective : To investigate the effect of taurine on amino acid neurotransmitters and their associated enzymes in rats with manganese - stained rats .


Methods : Sprague - Dawley rats were randomly divided into two groups : blank control group , manganese - stained group , prevention group , treatment group and control group .
The contents of glutamic acid ( Glu ) , glutamine ( glutamine , Gln ) and gamma - aminobutyric acid ( GABA ) were determined by high performance liquid chromatography ( HPLC ) UV detection method .
The contents of glutamate decarboxylase ( GAD ) , phosphoactivated glutamine ( PAG ) and glutamine synthetase ( GS ) were determined by enzyme linked immunosorbent assay ( ELISA ) .


Results : 1 . Compared with the control group , the number of escape latency in the treatment group was significantly shorter than that in the control group ( P0.05 ) . The number of escape latency in the treatment group was significantly shorter than that in the control group ( P0.05 ) .
Compared with the control group , the content of Glu in the treated group was higher than that of blank control group ( P0.05 ) .
The content of G1n and GABA in the exposed group was lower than that in the blank control group ( P0.05 ) .
The content of PAG in the manganese - stained group was higher than that in the blank control group ( P0.05 ) . The GAD and GS contents of the manganese - stained group were lower than those in the blank control group ( P0.05 ) .
When taurine was used for 8 weeks , the content of Glu in the prevention group was lower than that in the exposed group ( P0.05 ) .
The content of G1n and GABA in the prevention group was higher than that in the manganese - stained group ( P0.05 ) .
Compared with the control group , the content of Glu in the treatment group was lower than that in the control group ( P0.05 ) , and the content of G1n and GABA in the treatment group was higher than that in the control group ( P0.05 ) .
The content of PAG in the treatment group was lower than that in the control group , the content of GAD and GS in the treatment group was higher than that in the control group ( P0.05 ) . The Glu content in the treatment group was lower than that in the control group ( P0.05 ) .
The content of G1n and GABA in the treatment group was higher than that in the manganese - stained group ( P0.05 ) . The content of PAG in the treatment group was lower than that of the manganese - stained group ( P0.05 ) . The GAD and GS contents in the treatment group were higher than those in the manganese - stained group ( P0.05 ) .


Conclusion : The changes of Glu , Gln and GABA in the hypothalamus of rats induced by manganese can increase , Gln and GABA decrease , taurine has an interference effect on the changes of Glu , Gln and GABA in the thalamus induced by manganese .
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R114;R151

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