發(fā)布時(shí)間：2018-05-23 14:22

  本文選題：益生菌 + 副干酪乳桿菌N�。� 參考：《營(yíng)養(yǎng)學(xué)報(bào)》2016年01期

【摘要】：目的研究副干酪乳桿菌(Lactobacillus paracasei,Lp)N1115對(duì)小鼠生命早期腸道發(fā)育的影響。方法 C57BL/6小鼠出生后D1,開(kāi)始喂食Lp N1115,檢測(cè)體質(zhì)量變化,至D14處死,用免疫組化法對(duì)絨毛寬度和隱窩深度,腸道上皮細(xì)胞增殖(Ki67核抗原)、分化(MUC2黏蛋白),緊密連接(ZO-1蛋白)進(jìn)行測(cè)定。結(jié)果與對(duì)照組比較,Lp N1115干預(yù)組小鼠體質(zhì)量在4 d后增加較快,體質(zhì)量變化有顯著性差異(P0.05)。免疫組化的結(jié)果顯示,Lp N1115干預(yù)組小鼠的絨毛密度和隱窩深度增加,同時(shí),K67陽(yáng)性細(xì)胞和MUC2陽(yáng)性細(xì)胞的數(shù)量較多,均有顯著性(P0.05,P0.05)。ZO-1免疫組化結(jié)果顯示,Lp N1115干預(yù)顯著增加了ZO-1膜定位表達(dá)(P0.05)。結(jié)論早期應(yīng)用Lp N1115可以促進(jìn)腸上皮細(xì)胞增殖、分化及腸粘膜功能的建立,促進(jìn)小鼠腸道發(fā)育。
[Abstract]:Objective to study the effect of Lactobacillus paracaseii LpN1115 on early intestinal development in mice. Methods C57BL/6 mice were fed with LpN1115 after birth and were killed by D14. The width of villi and the depth of crypt were determined by immunohistochemical method. The proliferation of intestinal epithelial cells was determined by Ki67 nuclear antigen, differentiation of MUC2 mucin and tight junction of ZO-1 protein. Results compared with the control group, the body weight of LpN1115 intervention group increased more rapidly after 4 days, and there was a significant difference in body mass change between LpN1115 group and control group (P 0.05). The results of immunohistochemistry showed that the density of villi and the depth of crypt were increased in LpN1115 group, and the number of K67 positive cells and MUC2 positive cells were more than that in LpN1115 group. The results of immunohistochemistry showed that LpN1115 significantly increased the expression of ZO-1 membrane. Conclusion early application of LP N1115 can promote the proliferation and differentiation of intestinal epithelial cells and the establishment of intestinal mucosal function, and promote the development of intestinal tract in mice.
【作者單位】： 石家莊君樂(lè)寶乳業(yè)有限公司;美國(guó)范德堡大學(xué)醫(yī)學(xué)中心;四川大學(xué)華西公共衛(wèi)生學(xué)院;
【基金】：河北省科技廳資助項(xiàng)目(No.14047104Z,159676219D,15227111D)
【分類號(hào)】：R151

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