目的探討汽油添加劑甲基叔丁基醚（methyl tert-butyl ether,MTBE）對(duì)人DNA聚合酶β低表達(dá)細(xì)胞的DNA損傷情況。方法運(yùn)用RNA干擾技術(shù)構(gòu)建人支氣管上皮細(xì)胞（HBE）DNA聚合酶β（polβ）低表達(dá)細(xì)胞株HBE-polβ^-;常規(guī)培養(yǎng)HBE-polβ^-、pEGFP-C1空載體細(xì)胞及HBE細(xì)胞至對(duì)數(shù)生長(zhǎng)期,分別以終濃度為0、2、4、8和16μmol/L的MTBE染毒培養(yǎng)48 h;以單細(xì)胞凝膠電泳（SCGE）檢測(cè)各組細(xì)胞的DNA損傷情況。結(jié)果 HBE-polβ^-細(xì)胞中polβ表達(dá)僅相當(dāng)于正常HBE細(xì)胞的18.38%;當(dāng)MTBE染毒濃度為2、4、8和16μmol/L時(shí),各組細(xì)胞均出現(xiàn)明顯的彗星拖尾現(xiàn)象,且HBE-polβ^-組細(xì)胞DNA彗星Olive尾矩與對(duì)照組比較,差異具有統(tǒng)計(jì)學(xué)意義（P<0.05）。結(jié)論 polβ的異常表達(dá)對(duì)MTBE所致DNA損傷可能易感。 

【文章來(lái)源】：毒理學(xué)雜志. 2020,34(04)CSCD

【文章頁(yè)數(shù)】：5 頁(yè)

【部分圖文】：

HBE-polβ-細(xì)胞、轉(zhuǎn)染“pEGFP-C1”空載體細(xì)胞和HBE細(xì)胞形態(tài)學(xué)觀察(×100)

各組細(xì)胞polβ表達(dá)的Western blot分析結(jié)果

以SCGE實(shí)驗(yàn)分析不同濃度的MTBE對(duì)HBE-polβ-細(xì)胞、轉(zhuǎn)染“pEGFP-C1”空載體細(xì)胞及HBE細(xì)胞的DNA損傷情況,結(jié)果發(fā)現(xiàn),當(dāng)MTBE染毒濃度為2、4、8和16 μmol/L時(shí),各組細(xì)胞均出現(xiàn)明顯的彗星拖尾現(xiàn)象,HBE-polβ-細(xì)胞彗星尾矩與對(duì)照組比較,差異有統(tǒng)計(jì)學(xué)意義;當(dāng)MTBE染毒濃度為16 μmol/L時(shí),各組細(xì)胞的DNA損傷最強(qiáng)(圖3)。各組細(xì)胞DNA損傷詳細(xì)情況如圖4所示。圖4 各組細(xì)胞DNA彗星尾矩

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