【摘要】：目的檢測早老素(Presenilinl,PS1)在局灶性腦缺血大鼠海馬組織神經(jīng)干細(xì)胞中的表達(dá)及穿梭箱訓(xùn)練的干預(yù)作用,探討影響神經(jīng)干細(xì)胞增殖分化的分子調(diào)控機(jī)制。方法采用大腦中動(dòng)脈栓塞(MCAO)制作局灶性腦缺血大鼠模型,用免疫組織化學(xué)方法檢測海馬神經(jīng)干細(xì)胞Brd U、PS1的表達(dá)及穿梭箱訓(xùn)練的干預(yù)作用。結(jié)果隨著腦缺血再灌注3 d缺血海馬神經(jīng)元Brd U陽性細(xì)胞明顯增多,7 d達(dá)高峰。PS1反應(yīng)產(chǎn)物腦缺血再灌注7 d較正常組增多,隨缺血再灌注時(shí)間的進(jìn)一步延長逐漸減少,14 d后持續(xù)低水平表達(dá)。穿梭箱訓(xùn)練后Brd U、PS1蛋白的表達(dá)明顯增加。結(jié)論穿梭箱訓(xùn)練促進(jìn)神經(jīng)干細(xì)胞的增殖及缺血腦組織PS1的表達(dá),提示穿梭箱訓(xùn)練促進(jìn)神經(jīng)干細(xì)胞增殖作用可能由PS1信號(hào)介導(dǎo)。
[Abstract]:Objective to detect the expression of Presenilinl,PS1 in hippocampal neural stem cells of rats with focal cerebral ischemia and the intervention effect of shuttle box training, and to explore the molecular regulation mechanism of neural stem cells proliferation and differentiation. Methods the rat model of focal cerebral ischemia was established by middle cerebral artery embolism (MCAO). The expression of Brd U and PS1 in hippocampal neural stem cells and the intervention effect of shuttle box training were detected by immunohistochemistry. Results the number of Brd U positive cells in ischemic hippocampal neurons increased significantly with 3 days of cerebral ischemia and reperfusion, and reached the peak at 7 days. PS1 reaction products increased after 7 days of cerebral ischemia and reperfusion, and decreased gradually with the further prolongation of ischemia and reperfusion time, and continued to low expression after 14 days. The expression of PS1 protein increased significantly after shuttle box training. Conclusion shuttle box training promotes the proliferation of neural stem cells and the expression of PS1 in ischemic brain tissue, suggesting that the effect of shuttle box training on the proliferation of neural stem cells may be mediated by PS1 signal.
【作者單位】： 沈陽醫(yī)學(xué)院解剖學(xué)教研室;沈陽醫(yī)學(xué)院病理學(xué)教研室;
【基金】：遼寧省科技廳科學(xué)技術(shù)計(jì)劃資助項(xiàng)目(2013020216)
【分類號(hào)】：R96

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