【摘要】：目的檢測早老素(Presenilinl,PS1)在局灶性腦缺血大鼠海馬組織神經(jīng)干細胞中的表達及穿梭箱訓(xùn)練的干預(yù)作用,探討影響神經(jīng)干細胞增殖分化的分子調(diào)控機制。方法采用大腦中動脈栓塞(MCAO)制作局灶性腦缺血大鼠模型,用免疫組織化學(xué)方法檢測海馬神經(jīng)干細胞Brd U、PS1的表達及穿梭箱訓(xùn)練的干預(yù)作用。結(jié)果隨著腦缺血再灌注3 d缺血海馬神經(jīng)元Brd U陽性細胞明顯增多,7 d達高峰。PS1反應(yīng)產(chǎn)物腦缺血再灌注7 d較正常組增多,隨缺血再灌注時間的進一步延長逐漸減少,14 d后持續(xù)低水平表達。穿梭箱訓(xùn)練后Brd U、PS1蛋白的表達明顯增加。結(jié)論穿梭箱訓(xùn)練促進神經(jīng)干細胞的增殖及缺血腦組織PS1的表達,提示穿梭箱訓(xùn)練促進神經(jīng)干細胞增殖作用可能由PS1信號介導(dǎo)。
[Abstract]:Objective to detect the expression of Presenilinl,PS1 in hippocampal neural stem cells of rats with focal cerebral ischemia and the intervention effect of shuttle box training, and to explore the molecular regulation mechanism of neural stem cells proliferation and differentiation. Methods the rat model of focal cerebral ischemia was established by middle cerebral artery embolism (MCAO). The expression of Brd U and PS1 in hippocampal neural stem cells and the intervention effect of shuttle box training were detected by immunohistochemistry. Results the number of Brd U positive cells in ischemic hippocampal neurons increased significantly with 3 days of cerebral ischemia and reperfusion, and reached the peak at 7 days. PS1 reaction products increased after 7 days of cerebral ischemia and reperfusion, and decreased gradually with the further prolongation of ischemia and reperfusion time, and continued to low expression after 14 days. The expression of PS1 protein increased significantly after shuttle box training. Conclusion shuttle box training promotes the proliferation of neural stem cells and the expression of PS1 in ischemic brain tissue, suggesting that the effect of shuttle box training on the proliferation of neural stem cells may be mediated by PS1 signal.
【作者單位】： 沈陽醫(yī)學(xué)院解剖學(xué)教研室;沈陽醫(yī)學(xué)院病理學(xué)教研室;
【基金】：遼寧省科技廳科學(xué)技術(shù)計劃資助項目(2013020216)
【分類號】：R96

【共引文獻】

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1 劉穎;張萱;劉純巖;倪文杰;毛洪濤;王珍琦;;抗精神病藥奎硫平對大鼠海馬神經(jīng)元死亡和細胞周期的影響[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2014年01期

2 齊亞超;肖向建;段瑞生;岳月紅;閆莉;靳瑋;張曉玲;;不同飼養(yǎng)環(huán)境對局灶性腦梗死大鼠梗死灶周圍VEGF表達的影響[J];腦與神經(jīng)疾病雜志;2013年05期

3 TAYLOR Chanel J;HE RongQiao;BARTLETT Perry F;;The role of the N-methyl-D-aspartate receptor in the proliferation of adult hippocampal neural stem and precursor cells[J];Science China(Life Sciences);2014年04期

4 王云杰;;高血壓腦出血患者的早期心理及肢體康復(fù)治療[J];內(nèi)蒙古醫(yī)學(xué)雜志;2014年01期

5 韋琛靜;周小青;劉旺華;李花;謝夢洲;張利美;李路迢;;丹龍醒腦方對局灶性腦缺血大鼠CA1區(qū)Nestin和GFAP表達的影響[J];湖南中醫(yī)藥大學(xué)學(xué)報;2014年05期

6 夏翠萍;陳進;沈麗華;;奧拉西坦對腦梗死大鼠細胞凋亡及神經(jīng)再生的影響[J];南通大學(xué)學(xué)報(醫(yī)學(xué)版);2014年03期

7 楊達;曾憲容;;卒中后大腦可塑性的研究進展[J];中國腦血管病雜志;2011年04期

8 王欣欣;馬珊珊;楊波;關(guān)方霞;;視知覺學(xué)習(xí)訓(xùn)練對海馬神經(jīng)細胞可塑性的影響及學(xué)習(xí)記憶功能的調(diào)控[J];鄭州大學(xué)學(xué)報(醫(yī)學(xué)版);2014年05期

9 Yun Xiang;Huihua Liu;Tiebin Yan;Zhiqiang Zhuang;Dongmei Jin;Yuan Peng;;Functional electrical stimulation-facilitated proliferation and regeneration of neural precursor cells in the brains of rats with cerebral infarction[J];Neural Regeneration Research;2014年03期

10 覃麗;郭忠信;楊伯寧;;豐富環(huán)境對錳中毒小鼠學(xué)習(xí)記憶能力和神經(jīng)發(fā)生的影響[J];神經(jīng)解剖學(xué)雜志;2014年01期

相關(guān)博士學(xué)位論文 前7條

1 喬陽;唾液酸與嬰兒生長發(fā)育的研究[D];蘇州大學(xué);2013年

2 許智祥;糖原合酶激酶3β在嗅覺系統(tǒng)中功能的研究[D];中國科學(xué)院研究生院（武漢物理與數(shù)學(xué)研究所）;2013年

3 邱e，

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