【摘要】：目的研究明日葉查爾酮(Angelica keiskei chalcones,AC)對(duì)胰島素抵抗L6細(xì)胞磷脂酰肌醇3激酶-蛋白激酶-葡萄糖轉(zhuǎn)運(yùn)體4(PI3K-Akt-GLUT4)信號(hào)通路的影響。方法采用高濃度胰島素誘導(dǎo)的胰島素抵抗L6細(xì)胞,分別加入5、10、20μmol/L終濃度的明日葉查爾酮共同培養(yǎng)24 h,葡萄糖氧化酶法測(cè)定細(xì)胞上清液中葡萄糖含量,逆轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)方法檢測(cè)細(xì)胞PI3K和Akt的mRNA表達(dá)水平,蛋白印跡法檢測(cè)GLUT4和Akt的蛋白表達(dá)水平。結(jié)果胰島素抵抗組細(xì)胞培養(yǎng)液的葡萄糖含量顯著高于空白對(duì)照組(P0.05),而PI3K mRNA、Akt mRNA和GLUT4、Akt蛋白表達(dá)水平則降低(P0.05);中、高劑量AC組細(xì)胞培養(yǎng)液葡萄糖含量均低于胰島素抵抗組(P0.05),而PI3K mRNA、Akt mRNA和GLUT4、Akt蛋白表達(dá)水平則升高(P0.05)。結(jié)論明日葉查爾酮可上調(diào)L6細(xì)胞胰島素抵抗模型PI3K-AktGLUT4信號(hào)通路的表達(dá)水平,增強(qiáng)其對(duì)葡萄糖的攝取利用,改善胰島素抵抗。
[Abstract]:Aim to study the effect of tomorrow Chalcone (Angelica keiskei chalcones,AC on phosphatidylinositol 3 kinase protein kinase glucose transporter 4 (PI3K-Akt-GLUT4) signaling pathway in insulin resistant L6 cells. Methods High concentration insulin induced insulin resistant L6 cells were co-cultured with 5 渭 mol / L, 10 渭 mol / L and 20 渭 mol / L tomorrow for 24 H. the glucose content in the culture medium was determined by glucose oxidase method, the mRNA expression of PI3K and Akt was detected by reverse transcriptase polymerase chain reaction (RT-PCR), and the protein expression of GLUT4 and Akt was detected by Western imprinting. Results the glucose content in the cell culture medium of insulin resistance group was significantly higher than that of blank control group (P 0.05), while the expression of PI3K mRNA,Akt mRNA and GLUT4,Akt protein was decreased (P 0.05). The glucose content of high dose AC group was lower than that of insulin resistance group (P 0.05), while the expression level of PI3K mRNA,Akt mRNA and GLUT4,Akt protein was increased (P 0.05). Conclusion tomorrow leaf chalcone can up-regulate the expression of PI3K-AktGLUT4 signaling pathway in L6 cells with insulin resistance, enhance its uptake and utilization of glucose, and improve insulin resistance.
【作者單位】： 廈門大學(xué)公共衛(wèi)生學(xué)院;中國(guó)人民解放軍總醫(yī)院;
【基金】：福建省自然科學(xué)基金計(jì)劃資助項(xiàng)目(No.2014J05098)
【分類號(hào)】：R96

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