【摘要】：目的觀察尾懸吊大鼠再負(fù)重骨量的變化及辛伐他汀干預(yù)對(duì)該過(guò)程的影響及機(jī)制。方法 5月齡大鼠24只分為4組,每組6只:正常對(duì)照組(CL組)、尾懸吊6周組(UL組)、尾懸吊3周再負(fù)重3周組(UL+RL組)、尾懸吊3周再負(fù)重加辛伐他汀干預(yù)3周組(10 mg/kg/d,UL+RL+SIM組);實(shí)驗(yàn)持續(xù)6周,處死大鼠取左側(cè)股骨進(jìn)行骨密度分析,取左側(cè)脛骨行骨組織形態(tài)計(jì)量學(xué)分析;取右側(cè)股骨經(jīng)生物力學(xué)試驗(yàn)分析最大載荷和彈性模量;取右側(cè)脛骨制備組織勻漿,提取RNA和蛋白,分別采用real-time PCR和western blot檢測(cè)Ⅰ型膠原(Col Ⅰ)的表達(dá)。結(jié)果 (1)骨密度:CL組顯著高于其余3組(P0.05),UL+RL組和UL+RL+SIM組均顯著高于UL組(P0.05);(2)骨組織形態(tài)計(jì)量學(xué):BV/TV:CL組顯著高于其余3組(P0.05),UL+RL組和UL+RL+SIM組均顯著高于UL組(P0.05);Tb.N:CL組顯著高于其余三組(P0.05);Tb.Th:CL組顯著高于UL組(P0.05);Tb.Sp:CL組顯著低于其余三組(P0.05),UL+RL組和UL+RL+SIM組均顯著低于UL組(P0.05)。(3)生物力學(xué)檢測(cè)結(jié)果:CL組最大載荷、彈性模量顯著高于其他3組(P0.05)。(4)Realtime PCR檢測(cè)結(jié)果:各組間ColⅠ的mRNA表達(dá)水平無(wú)顯著差別。(5)Western blot:UL組ColⅠIOD值顯著低于CL組(P0.05),其余組間差異無(wú)顯著性。結(jié)論大鼠尾懸吊誘發(fā)下肢骨量丟失、微結(jié)構(gòu)退變、力學(xué)性能下降、Ⅰ型膠原含量減少,而再負(fù)重后上述指標(biāo)可得到部分恢復(fù),辛伐他汀干預(yù)不能促進(jìn)這一過(guò)程。
[Abstract]:Objective to observe the changes of reload bone mass in tail suspended rats and the effect and mechanism of simvastatin intervention on this process. Methods Twenty-four 5-month-old rats were divided into 4 groups with 6 rats in each group: normal control group (CL group), tail suspension group (UL RL group) for 3 weeks, tail suspension group for 3 weeks plus simvastatin intervention group (10 mg/kg/d,UL RL SIM group), the rats were sacrificed for bone mineral density analysis, and the left tibia was taken for bone histomorphology analysis. The maximum load and elastic modulus of the right thigh were analyzed by biomechanical test, and the tissue homogenate was prepared from the right tibia, RNA and protein were extracted, and the expression of type I collagen (Col 鈪，

本文編號(hào)：2500971