【摘要】：目的研究高遷移率族蛋白1(HMGB1)抗體對刀豆蛋白A(ConA)引起小鼠肝損傷的保護作用。方法將健康無污染雄性Balb/c小鼠分成空白對照組(注射生理鹽水)、模型組(注射ConA)和實驗組(注射ConA+HMGB1抗體),3組小鼠注射藥物6 h后摘眼球取血,所獲血液離心后取血清檢測谷丙轉(zhuǎn)氨酶(ALT)和HMGB1,留取肝臟組織進行HE染色、Tunel、免疫熒光等檢測。結(jié)果實驗組小鼠肝組織病理炎癥反應(yīng)輕于模型組。小鼠血清中ALT、HMGB1:空白對照組分別為(52.00±8.34)U/L、(7.54±0.53)ng/mL,模型組分別為(5 551.50±1 445.74)U/L、(18.06±1.65)ng/mL,實驗組分別為(1 977.40±654.89)U/L、(10.77±0.71)ng/mL;肝組織中HMGB1 mRNA、HMGB1表達量(相對值):空白對照組分別為1.886±0.253、0.086±0.028,模型組分別為4.718±0.341、0.268±0.043,實驗組分別為3.005±0.331、0.116±0.008;實驗組小鼠血清中ALT、HMGB1,以及肝組織中HMGB1 mRNA、HMGB1表達量均低于模型組(均P0.001)。肝組織細胞凋亡、肝細胞內(nèi)HMGB1遷移(標(biāo)準(zhǔn)化):空白對照組均為1±0,模型組分別為4.67±0.33、4.50±0.22,實驗組分別為2.67±0.21、2.33±0.21;實驗組小鼠肝組織細胞凋亡、肝細胞內(nèi)HMGB1遷移均低于模型組(均P0.001)。結(jié)論 HMGB1抗體能改善肝組織的病理損傷,可以保護ConA引起的小鼠肝損傷。
[Abstract]:Objective to study the protective effect of high mobility group protein 1 (HMGB1) antibody on liver injury induced by concanavalin A (ConA) in mice. Methods healthy unpolluted male Balb/c mice were divided into three groups: blank control group (injected with normal saline), model group (injected with ConA) and experimental group (injected with ConA HMGB1 antibody). The eyeballs of the three groups were collected after 6 hours of injection. After centrifugation, serum samples were taken to detect glutamic-pyruvic transaminase (ALT) and HMGB1, for HE staining and Tunel, immunofluorescence. Results the pathological inflammatory reaction of liver tissue in the experimental group was lighter than that in the model group. The serum ALT,HMGB1: blank control group was (52.00 鹵8.34) U 鈮，

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