【摘要】：目的觀察碳酸二乙酯(DEC)對雌雄大鼠生育力與早期胚胎發(fā)育的影響。方法雌雄SD大鼠分別經(jīng)口給予DEC 80、200和500 mg/kg·bw·d及陰性對照(大豆油,10 ml/kg·d),雄鼠連續(xù)給藥4周、雌鼠連續(xù)給藥2周后按1∶1合籠,交配成功后雄鼠繼續(xù)給藥至剖解,雌鼠繼續(xù)給藥至妊娠第6天(GD6)。試驗期間觀察各組雌雄SD大鼠的一般毒性反應(yīng)、體重及攝食量影響,合籠期間雌鼠動情周期,記錄交配情況;交配成功雌鼠于GD15剖檢,進行終末檢查,評價雌性生殖機能、生育力,以及子代的胚胎形成和早期發(fā)育影響;雄鼠于交配成功后剖檢,并分析精子濃度及活力,評價雄性生殖機能、生育力影響。結(jié)果各劑量組DEC對雌雄大鼠親代均未出現(xiàn)明顯的親代一般毒副反應(yīng);對雌雄大鼠體重未見明顯異常,僅高劑量GD0和低劑量GD0、GD6雌鼠體重略低于陰性對照組(P0.05);對雌雄大鼠的攝食量、生殖系統(tǒng)臟器重量和臟器系數(shù)、交配成功天數(shù)、交配率、妊娠率、雌鼠動情周期、雄鼠精子濃度及活力均未見明顯影響;對孕鼠子代胚胎形成和早期發(fā)育未見明顯影響。結(jié)論在本試驗條件下,雌雄SD大鼠的生育力以及早期胚胎發(fā)育均未見明顯毒性影響,未觀察到明顯毒性反應(yīng)劑量(NOEAL)為500 mg/kg·bw·d。
[Abstract]:Objective to observe the effect of diethyl carbonate (DEC) on fertility and early embryonic development in male and female rats. Methods male and female SD rats were given DEC 80200 and 500 mg/ kg 路bw 路d and negative control (soybean oil, 10 ml/ kg 路d), male rats for 4 weeks). The female rats were given DEC 80200 and 500 mg/ kg 路d), 路d respectively for 4 weeks, and the female rats were caged at 1:1 after 2 weeks of continuous administration. After successful mating, the male rats continued to be given until the dissecting, and the female mice continued to be given until the 6th day of pregnancy (GD6). During the experiment, the general toxic reaction, body weight and food intake of male and female SD rats were observed, the estrous cycle of female rats during cage period was observed, and the mating situation was recorded. The successful mating female mice were examined by GD15, and the female reproductive function, fertility, embryo formation and early development of offspring were evaluated by terminal examination. The male mice were examined after successful mating, and the sperm concentration and motility were analyzed to evaluate the effects of male reproductive function and fertility. Results DEC in each dose group had no obvious toxic and side effects on the parents of male and female rats, but there was no significant abnormality in the body weight of male and female rats, but the body weight of female rats with high dose GD0 and low dose GD0,GD6 was slightly lower than that of negative control group (P 0.05). There were no significant effects on food intake, reproductive system organ weight and organ coefficient, mating success days, mating rate, pregnancy rate, estrus cycle, sperm concentration and motility of male and female rats. There was no significant effect on embryo formation and early development of pregnant rats. Conclusion under the experimental conditions, there is no obvious toxic effect on fertility and early embryonic development in male and female SD rats, and the obvious toxic reaction dose (NOEAL) is 500 mg/ kg 路bw 路d.
【作者單位】： 浙江大學(xué)藥學(xué)院;浙江省醫(yī)學(xué)科學(xué)院安全性評價研究中心;
【基金】：浙江省科技計劃項目(2014F30018) 浙江省醫(yī)學(xué)科學(xué)青年基金
【分類號】：R965

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