【摘要】：目的觀察40和200 nm 2種粒徑納米氧化銅顆粒(Cu ONPs)對豚鼠血腦屏障通透性造成的影響。方法制備粒徑為40和200 nm的近球型Cu ONPs用于測試。以0.5%HPMC作為分散劑配制Cu ONPs染毒分散懸液。選取250~300 g的豚鼠用于試驗(yàn)。試驗(yàn)設(shè)40和200 nm Cu ONPs組、Cu SO4組、0.5%HPMC組和生理鹽水組,每組5只動(dòng)物。以一次性舌下靜脈注射方式染毒。Cu ONPs組、Cu SO4組、HPMC組和生理鹽水組染毒劑量分別按照1.5、65、4和4 ml/kg·bw進(jìn)行。分別于染毒后24和48 h從每組取5只動(dòng)物,先經(jīng)舌下靜脈給動(dòng)物注射2%伊文氏藍(lán)(EB)生理鹽水溶液(4 ml/kg),1 h后實(shí)施心臟灌注,切取大腦頂葉皮質(zhì)及皮質(zhì)下結(jié)構(gòu)組織,以腦組織中EB滲入量(以吸光度A值計(jì))表示動(dòng)物血腦屏障的開放程度。結(jié)果 40 nm Cu ONP作用24和48 h時(shí)皮質(zhì)和皮質(zhì)下結(jié)構(gòu)組織A值明顯高于相應(yīng)200 nm Cu ONP組和生理鹽水組A值差異有統(tǒng)計(jì)學(xué)意義(P0.05)。2種粒徑Cu ONPs組作用48 h的A值較各自作用24 h的A值明顯增大差異有統(tǒng)計(jì)學(xué)意義(P0.05)。同時(shí),200 nm Cu ONPs組48 h皮質(zhì)A值(0.383±0.017 A/mg)與生理鹽水組A值(0.103±0.021 A/mg)之間差異有統(tǒng)計(jì)學(xué)意義(P0.05)。除Cu SO4組24和48 h皮質(zhì)下結(jié)構(gòu)A值分別為0.087±0.026和0.071±0.031 A/mg高于對應(yīng)生理鹽水組,分別為0.041±0.013和0.069±0.022 A/mg外,其余各組的A值與生理鹽水組間差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論 40 nm Cu ONP具有顯著的致豚鼠血腦屏障開放效應(yīng),且這種效應(yīng)在作用24~48 h具有增強(qiáng)特點(diǎn)。
[Abstract]:Objective to observe the effect of 40 and 200 nm nano-sized copper oxide particles (Cu ONPs) on the permeability of blood-brain barrier in guinea pigs. Methods the near spherical Cu ONPs with particle size of 40 nm and 200 nm was prepared for testing. The dispersion suspension of Cu ONPs was prepared by using 0.5%HPMC as dispersant. A guinea pig of 250 g 300 g was selected for the experiment. Five animals in each group were divided into four groups: 40 and 200 nm Cu ONPs groups, Cu SO4 group, 0.5%HPMC group and saline group. . Cu ONPs group, Cu SO4 group, HPMC group and saline group were administrated according to 1.5 ml/ kg 路bw, 65 ml/ kg 路bw, 4 ml/ kg 路bw and 4 ml/ kg 路bw, respectively. Five animals in each group were taken from each group at 24 and 48 h after exposure. The animals were injected with 2% Evans blue (EB) saline solution (4 ml/kg) through the sublingual vein, and then cardiac perfusion was performed 1 hour later. The parietal lobe cortex and subcortical structure tissue were removed and the opening degree of blood-brain barrier (BBB) was indicated by the amount of EB infiltration in brain tissue (measured by absorbance A value). Results the A value of cortex and subcortical structure tissue exposed to 40 nm Cu ONP for 24 and 48 h was significantly higher than that of the corresponding 200 nm Cu ONP group and physiological saline group (P0.05). The A values of the two Cu ONPs groups treated for 48 h were significantly higher than those of the control group (P0.05). There was significant difference in A value between the two groups at 24 h (P0.05). At the same time, the cortical A value of 200 nm Cu ONPs group (0.383 鹵0.017 A/mg) was significantly different from that of physiological saline group (0.103 鹵0.021 A/mg) (P0.05). Except the A value of subcortical structure in Cu SO4 group was 0.087 鹵0.026 and 0.071 鹵0.031 A/mg higher than that in normal saline group (0.041 鹵0.013 and 0.069 鹵0.022 A/mg) at 24 and 48 hours, respectively. There was no significant difference between the other groups in A value and saline group (P0.05). Conclusion 40 nm Cu ONP has a significant open effect on blood-brain barrier in guinea pigs, and this effect can be enhanced at 24 h and 48 h.
【作者單位】： 中國疾病預(yù)防控制中心環(huán)境與健康相關(guān)產(chǎn)品安全所環(huán)境毒理室;北京工業(yè)大學(xué)環(huán)境與能源工程學(xué)院;
【基金】：國家自然科學(xué)基金(81372949)
【分類號(hào)】：R99

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