發(fā)布時間：2019-04-02 03:40

【摘要】：研究關附甲素(Guanfu base A,GFA)的抗房顫和抗氧化作用。大鼠尾靜脈注射乙酰膽堿-氯化鈣混合液(Ca Cl210 mg/m L,Ach 66μg/m L),連續(xù)7 d建立房顫模型。SD大鼠分為正常組、模型組、GFA治療組(6 mg/kg,12 mg/kg)、胺碘酮(Amiodarone,Ami)治療組(50 mg/kg)以及洛伐他汀(Lovastatin,Lov)治療組(10 mg/kg)。測定大鼠房顫持續(xù)時間和有效不應期(AERP),用實時熒光定量PCR方法和Western blot方法測定氧化應激相關基因表達,試劑盒測定抗氧化酶的活性。結果顯示:與模型組相比,GFA(6 mg/kg,12 mg/kg,po)治療4 d后能縮短房顫持續(xù)時間,延長AERP,超氧化物歧化酶的活力提高,丙二醛含量明顯下降。心房肌p22phox、p47phox、p67phox、gp91phox表達下調,膜Rac-1與胞漿Rac-1比值顯著下降,縫隙連接蛋白(connexin40)表達升高。提示GFA(6 mg/kg,12 mg/kg)能有效抑制房顫引起的大鼠心房氧化應激損害,抑制房顫發(fā)生。
[Abstract]:To study the anti-atrial fibrillation and antioxidation effects of Guanfu A (Guanfu base A). The rat model of atrial fibrillation was established by intravenous injection of acetylcholine-calcium chloride mixed solution (Ca Cl210 mg/m L, Ach 66ug / m L),) for 7 days. SD rats were divided into normal group, model group and GFA treatment group (6 mg/kg,12 mg/kg). Amiodarone (Amiodarone,Ami) group (50 mg/kg) and lovastatin (Lovastatin,Lov) group (10 mg/kg). The duration of atrial fibrillation (AF) and the effective refractory period (AERP),) of rats were measured by real-time fluorescence quantitative PCR and Western blot, and the activity of antioxidant enzymes was measured by Elisa. The results showed that compared with the model group, GFA (6 mg/kg,12 mg/kg,po could shorten the duration of atrial fibrillation, prolong the activity of AERP, superoxide dismutase (SOD) and decrease the content of malondialdehyde (MDA). The expression of p22phoxs, p47phoxs, p67phoxs and gp91phox was down-regulated, the ratio of membrane Rac-1 to cytoplasmic Rac-1 was significantly decreased, and the expression of gap junction protein (connexin40) was increased. It is suggested that GFA (6 mg/kg,12 mg/kg) can effectively inhibit atrial oxidative stress damage induced by atrial fibrillation and inhibit the occurrence of atrial fibrillation in rats.
【作者單位】： 中國藥科大學臨床藥學教研室;
【基金】：國家“重大新藥創(chuàng)制”科技重大專項課題資助項目(No.2009ZX09103-345)~~
【分類號】：R965

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