24孔板培養(yǎng)活躍鏈霉菌合成那西肽條件的優(yōu)化
發(fā)布時(shí)間：2019-03-09 09:37
【摘要】：目的優(yōu)化24孔板培養(yǎng)活躍鏈霉菌XW產(chǎn)那西肽的發(fā)酵條件。方法采用單因素實(shí)驗(yàn)和響應(yīng)面法對24孔板的發(fā)酵條件進(jìn)行優(yōu)化。結(jié)果得到活躍鏈霉菌XW 24孔板發(fā)酵產(chǎn)那西肽的最優(yōu)發(fā)酵條件為:種子培養(yǎng)基裝液量2.55 m L,發(fā)酵培養(yǎng)基裝液量0.93m L,發(fā)酵時(shí)間121h。結(jié)論通過對發(fā)酵條件的優(yōu)化,可以明顯提高24孔板發(fā)酵產(chǎn)那西肽的效價(jià)達(dá)到882.25μg/m L,實(shí)驗(yàn)值與模型預(yù)測值較接近,吻合度較高,較初始效價(jià)675.63μg/m L提高了30.58%。研究結(jié)果表明24孔板發(fā)酵與搖瓶發(fā)酵之間有良好的相關(guān)性,相關(guān)系數(shù)R為0.883。
[Abstract]:Objective to optimize the fermentation conditions of natoxin produced by Streptomyces XW in 24-well plate culture. Methods single factor experiment and response surface method were used to optimize the fermentation conditions of 24-well plate. Results the optimum fermentation conditions were as follows: the amount of seed culture medium was 2.55 mL, the amount of fermentation medium was 0.93m L, and the fermentation time was 121 h. The optimal fermentation conditions of Streptomyces actives XW 24-well plate fermentation were as follows: the amount of liquid in seed culture medium was 2.55 mL, the fermentation time was 121 h. Conclusion through the optimization of fermentation conditions, the titer of nacitide produced by 24-well plate fermentation reached 882.25 渭 g / mL, the experimental value was close to the predicted value of the model, and the coincidence degree was higher. Compared with the initial titer of 675.63 渭 g / mL, it was increased by 30.58%. The results showed that there was a good correlation between 24-well plate fermentation and shaking flask fermentation, and the correlation coefficient was 0.883.
【作者單位】： 安徽工程大學(xué)生物與化學(xué)工程學(xué)院;安徽省皖北藥業(yè)股份有限公司;
【基金】：安徽省高校自然科學(xué)基金(No.KJ2013A048)
【分類號】：R914.5
[Abstract]:Objective to optimize the fermentation conditions of natoxin produced by Streptomyces XW in 24-well plate culture. Methods single factor experiment and response surface method were used to optimize the fermentation conditions of 24-well plate. Results the optimum fermentation conditions were as follows: the amount of seed culture medium was 2.55 mL, the amount of fermentation medium was 0.93m L, and the fermentation time was 121 h. The optimal fermentation conditions of Streptomyces actives XW 24-well plate fermentation were as follows: the amount of liquid in seed culture medium was 2.55 mL, the fermentation time was 121 h. Conclusion through the optimization of fermentation conditions, the titer of nacitide produced by 24-well plate fermentation reached 882.25 渭 g / mL, the experimental value was close to the predicted value of the model, and the coincidence degree was higher. Compared with the initial titer of 675.63 渭 g / mL, it was increased by 30.58%. The results showed that there was a good correlation between 24-well plate fermentation and shaking flask fermentation, and the correlation coefficient was 0.883.
【作者單位】： 安徽工程大學(xué)生物與化學(xué)工程學(xué)院;安徽省皖北藥業(yè)股份有限公司;
【基金】：安徽省高校自然科學(xué)基金(No.KJ2013A048)
【分類號】：R914.5
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