【摘要】：目的研究丹那唑?qū)Υ笫笤渭毎膿p傷作用與機制。方法原代大鼠肝細胞貼壁培養(yǎng)4 h,加入丹那唑0,50,100,200μmol/L,4 h和24 h后采用乳酸脫氫酶法測定細胞活力并檢測三磷酸腺苷(ATP)水平,同時提取線粒體,分別測5個呼吸鏈復(fù)合酶(complex Ⅰ~V)活性。培養(yǎng)液中加入20 mmol/L果糖促進糖酵解,觀察果糖對丹那唑細胞毒性的影響。結(jié)果丹那唑50,100μmol/L處理4 h和24 h對細胞幾乎沒有殺傷作用,但可致ATP顯著降低;200μmol/L處理4 h和24 h均可導(dǎo)致細胞壞死。果糖可對抗丹那唑4 h具殺傷作用,但對24 h殺傷作用無效。丹那唑(50~200μmol/L)顯著抑制線粒體呼吸鏈復(fù)合酶Ⅰ活性,但不影響complexⅡ~V活性。結(jié)論丹那唑選擇性抑制線粒體呼吸鏈復(fù)合酶Ⅰ活性而導(dǎo)致ATP生成減少并誘發(fā)細胞壞死。
[Abstract]:Objective to study the damage effect and mechanism of danazol on primary rat hepatocytes. Methods Primary rat hepatocytes were cultured for 4 h, then added with danazol 050100200 渭 mol/L,4 / h and 24 h later, the cell viability and adenosine triphosphate (ATP) levels were measured by lactate dehydrogenase assay, and mitochondria were extracted simultaneously. The activities of five respiratory chain complex enzymes (complex 鈪，

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