【摘要】：10-脫乙酰基巴卡亭III(10-DAB)是紫杉烷類抗腫瘤藥物多烯紫杉醇的前體物質。采用響應面法優(yōu)化成團泛菌P2-A-8轉化巴卡亭III生成10-DAB的工藝條件。利用Box-Behnken試驗和方差分析,從菌體培養(yǎng)時間、菌體濃度OD600、底物終濃度、轉化溫度、轉化時間5個方面優(yōu)化成團泛菌P2-A-8轉化巴卡亭III生成10-DAB的工藝條件。結果表明,獲得最佳轉化工藝條件為:菌體經過二級培養(yǎng)12 h,轉接后調節(jié)初始OD600為3.0,此時,加入溶解于甲醇的巴卡亭III溶液,使其終濃度為0.007 mg/m L,32℃,200 r/min下轉化4 d,此時10-DAB的生成率達到24.5%,比優(yōu)化前提高了446.8%。實驗結果表明,響應面法優(yōu)化成團泛菌P2-A-8轉化巴卡亭III生成10-DAB的工藝條件合理可行。該研究成果對于多烯紫杉醇生物合成具有重要的應用價值。
[Abstract]:III (10-DAB) is a precursor of docetaxel, a taxane antitumor drug. Response surface method (RSM) was used to optimize the process conditions for the transformation of Vaca III into 10-DAB by panbacterium P2-A-8. Box-Behnken test and variance analysis were used to optimize the process conditions for the transformation of III from P2-A-8 to 10-DAB from five aspects: cell culture time, final concentration of OD600, substrate, transformation temperature and transformation time. The results showed that the optimum transformation conditions were as follows: after 12 hours of secondary culture, the initial OD600 was adjusted to 3.0, and the final concentration of Vaca III dissolved in methanol was 0.007 mg/m L ~ (2 +) at 32 鈩，

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