【摘要】：目的采用高效液相色譜法(HPLC)體外模擬體內(nèi)環(huán)境條件確定丙泊酚、依托咪酯混合后的新物質(zhì)生成情況。方法采用十八烷基硅烷鍵合硅膠作為填充劑(250.0mm×4.6mm,5μm),以磷酸鹽緩沖液-乙腈(調(diào)至pH7.4)作為流動(dòng)相[磷酸鹽緩沖液∶乙腈=35∶65(V/V)],設(shè)定柱溫為30℃,流速為1.0mL/min,進(jìn)樣量為10μL,全波長下進(jìn)行紫外光譜檢測(cè)(波長190~800nm),通過對(duì)比標(biāo)準(zhǔn)品及對(duì)照品圖譜確認(rèn)圖譜中是否有新的特征峰。結(jié)果通過與標(biāo)準(zhǔn)品圖譜進(jìn)行比較發(fā)現(xiàn),丙泊酚、依托咪酯二者混合品在體外模擬體內(nèi)環(huán)境條件下無新的特征峰出現(xiàn),即未生成第3種物質(zhì)。結(jié)論所建方法簡便、快速、準(zhǔn)確地檢測(cè)出丙泊酚、依托咪酯混合后無新物質(zhì)生成。
[Abstract]:Objective to determine the production of propofol and etomidate by (HPLC) in vitro. Methods Octadecyl silane bonded silica gel was used as filler (250.0mm 脳 4.6 mm ~ 5 渭 m),) and phosphate buffer solution and acetonitrile (pH7.4) were used as mobile phase [phosphate buffer: acetonitrile = 35:65 (V / V)]. The column temperature was set at 30 鈩，

本文編號(hào)：2391908