【摘要】：目的:研究一種制備粒徑均一可控的載蛋白緩釋微球的新工藝,探究微球粒徑、載體材料、形態(tài)結(jié)構(gòu)與微球載藥釋藥性能的關(guān)系。方法:以牛血清白蛋白(BSA)為模型藥物,PLGA和PEG-PLGA作為載體材料,采用SPG膜乳化法,通過調(diào)整不同孔徑(3,5,7,12μm)的SPG膜制備不同粒徑的微球�？疾煳⑶蛄�、包封率、釋放行為、表面/內(nèi)部形態(tài)等性質(zhì),并對微球微觀結(jié)構(gòu)相關(guān)的參數(shù)如孔徑、平面孔隙率等進行定量分析。結(jié)果:微球的粒徑與SPG膜孔徑呈正相關(guān)關(guān)系,且相關(guān)系數(shù)0.9。隨著微球粒徑的增大,載藥量和包封率也呈現(xiàn)增大的趨勢,突釋減輕。PLGA和PEG-PLGA微球的內(nèi)部結(jié)構(gòu)隨微球粒徑增加的變化差異較大。結(jié)論:獲得較為滿意的制備載蛋白微球的新工藝,微球形態(tài)圓整,粒徑均一可控。
[Abstract]:Aim: to study a new process for preparation of protein-loaded sustained-release microspheres with uniform and controllable particle size, and to explore the relationship between the particle size, carrier material, morphology and drug delivery properties of microspheres. Methods: bovine serum albumin (BSA) (BSA) was used as model drug, PLGA and PEG-PLGA were used as carrier materials, and SPG membrane emulsification method was used to prepare different diameter microspheres of SPG membrane with different pore size (3 ~ 5 ~ 712 渭 m). The particle size, encapsulation efficiency, release behavior, surface / internal morphology of the microspheres were investigated. The parameters related to the microstructure of the microspheres, such as pore size and planar porosity, were analyzed quantitatively. Results: the diameter of microspheres was positively correlated with the pore size of SPG membrane, and the correlation coefficient was 0.9. With the increase of the particle size of the microspheres, the drug loading and entrapment efficiency increased, and the sudden release decreased. The internal structure of PLGA and PEG-PLGA microspheres varied greatly with the increase of the particle size. Conclusion: the new technology of preparing protein loaded microspheres is satisfactory. The microspheres are round in shape and uniform in size.
【作者單位】： 中山大學(xué)藥學(xué)院;中山大學(xué)附屬第三醫(yī)院藥劑科;
【分類號】：R943
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本文編號：2390640