【摘要】：目的研究亞抑菌濃度頭孢他啶對臨床尿管分離大腸埃希菌生物膜形成的作用。方法采用瓊脂平板倍比稀釋法檢測大腸埃希菌的最低抑菌濃度,雙紙片協(xié)同法檢測大腸埃希菌產(chǎn)超廣譜β內(nèi)酰胺酶(extended spectrum beta-lactamases,ESBLs)的能力,菌落計數(shù)法分析細菌的粘附能力,96孔板結(jié)晶紫染色法檢測細菌生物膜的形成。結(jié)果臨床分離大腸埃希菌對氨芐西林、頭孢哌酮、頭孢噻肟和哌拉西林具有較高的耐藥性,對阿米卡星、亞胺培南和美洛培南全部敏感。大腸埃希菌ESBLs陽性率為73.3%。1/4 MIC頭孢他啶對大腸埃希菌生物膜形成均有抑制作用,生物膜形成能力降低8.7%~58.0%。除了大腸埃希菌E11和E15差異不顯著外(P0.05),亞抑菌濃度頭孢他啶對其他14株菌的生物膜均有顯著的抑制效果(P0.05)。結(jié)論亞抑菌濃度頭孢他啶可抑制臨床尿管分離的大腸埃希菌生物膜形成,但其抑制程度具有菌株差異性。
[Abstract]:Objective to study the effect of ceftazidime on biofilm formation of Escherichia coli isolated from clinical urinary catheter. Methods the minimal inhibitory concentration of Escherichia coli and the ability of producing extended-spectrum 尾 -lactamase (extended spectrum beta-lactamases,ESBLs were detected by Agar plate dilution method and double disk synergy method. The adhesion ability of bacteria was analyzed by colony counting method and the formation of bacterial biofilm was detected by 96 hole plate crystal violet staining. Results the clinical isolates of Escherichia coli showed high resistance to ampicillin, cefoperazone, cefotaxime and piperacillin, and were all sensitive to amikacin, imipenem and meropenem. The ESBLs positive rate of Escherichia coli was 73.3%. 1 / 4 MIC ceftazidime inhibited the biofilm formation of Escherichia coli, and the ability of biofilm formation decreased by 8.7% and 58.0%. Except Escherichia coli E11 and E15, ceftazidime had significant inhibitory effect on the biofilm of other 14 strains (P0.05). Conclusion Ceftazidime can inhibit the biofilm formation of Escherichia coli isolated by clinical urinary catheter, but its inhibition degree is different.
【作者單位】： 第三軍醫(yī)大學西南醫(yī)院藥劑科;解放軍第302醫(yī)院肝硬化診療中心;第三軍醫(yī)大學西南醫(yī)院泌尿外科;
【分類號】：R915
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本文編號：2386713