【摘要】：建立凝血酶凍干粉分子量分布測(cè)定方法。色譜柱為蛋白專用柱TSK GEL G2000 SW(7.5mm×300mm,10μm),以p H6.86磷酸鹽緩沖液為流動(dòng)相,流速0.5m L/min,紫外檢測(cè)器,波長(zhǎng)280nm。凝血酶凍干粉重均分子量為80ku~95ku。所建立的凝血酶凍干粉分子量測(cè)定方法具有快速、簡(jiǎn)便和重復(fù)性好的特點(diǎn),為提高和完善制劑的質(zhì)量標(biāo)準(zhǔn)提供了依據(jù),同時(shí)可作為特征圖譜從蛋白分子量角度來控制該產(chǎn)品的內(nèi)在質(zhì)量。
[Abstract]:A method for determination of molecular weight distribution of thrombin freeze-dried powder was established. The chromatographic column was TSK GEL G2000 SW (7.5mm 脳 300mm ~ (-1) 10 渭 m),) with pH 6.86 phosphate buffer as mobile phase, flow rate was 0.5 mL / min, UV detector, wavelength was 280 nm. The average molecular weight of thrombin freeze-dried powder is 80ku-95ku. The established method for the determination of molecular weight of thrombin freeze-dried powder is rapid, simple and reproducible, which provides a basis for improving and improving the quality standard of the preparation. At the same time, it can be used as characteristic map to control the internal quality of the product from the angle of protein molecular weight.
【作者單位】： 吉林省中醫(yī)藥科學(xué)院;吉林大學(xué)第一醫(yī)院;
【基金】：吉林省科技發(fā)展計(jì)劃項(xiàng)目(201503018YY)
【分類號(hào)】：R927.11;O657.72

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