【摘要】：建立了超高效液相色譜-串聯(lián)質(zhì)譜法(UPLC-MS/MS)測定犬血漿中利培酮和帕潘立酮的濃度。血漿樣品用乙腈沉淀蛋白后,用Zorbax SB-C18柱分離,以乙腈-10mmol/L乙酸銨溶液(65∶35,V/V)為流動(dòng)相,在電噴霧離子源正離子模式下以多反應(yīng)監(jiān)測(MRM)方式進(jìn)行檢測。利培酮、帕潘立酮分別在0.05~30.0ng/mL和0.10~30.0ng/mL范圍內(nèi)線性關(guān)系良好(r~20.99),定量限分別為0.05ng/mL和0.10ng/mL,回收率分別為91.2%~95.1%和93.1%~97.5%。該方法精密、準(zhǔn)確、快速,適用于臨床血藥濃度的監(jiān)測及利培酮緩釋微球藥動(dòng)學(xué)的研究。
[Abstract]:A high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of risperidone and patridone in canine plasma. The plasma samples were precipitated with acetonitrile and separated by Zorbax SB-C18 column. The mobile phase of acetonitrile-ammonium 10mmol/L acetate solution (65: 35 V / V) was determined by multi-reaction monitoring (MRM) under the positive ion mode of electrospray ion source. The linear relationship between risperidone and Papridol in the range of 0.05~30.0ng/mL and 0.10~30.0ng/mL was good (r = 20.99), and the quantification limits were 0.05ng/mL and 0.10ng / mL, respectively. The recoveries were 91.2% and 93.1%, respectively. The method is accurate, accurate and rapid. It is suitable for clinical monitoring of blood drug concentration and pharmacokinetics of risperidone sustained-release microspheres.
【作者單位】： 遼寧省分析科學(xué)研究院;吉林大學(xué)生命科學(xué)學(xué)院;
【分類號(hào)】：O657.63;R965

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