發(fā)布時間：2018-12-12 09:53

【摘要】：目的:探討左炔諾孕酮誘導體外人子宮肌瘤細胞凋亡的抗腫瘤機制。方法:單層細胞培養(yǎng)后,使用MTT法分析左炔諾孕酮對人子宮肌瘤細胞增殖作用;使用流式細胞術分析左炔諾孕酮對人子宮肌瘤細胞周期與凋亡的影響;使用Westernblot法分析左炔諾孕酮對人子宮肌瘤細胞P53凋亡通路的影響。結果:左炔諾孕酮對人子宮肌瘤細胞的增殖具有明顯抑制作用;左炔諾孕酮促使人子宮肌瘤細胞周期阻滯于G2/M期,并誘導細胞發(fā)生凋亡;左炔諾孕酮激活人子宮肌瘤細胞P53凋亡通路而引起細胞發(fā)生凋亡。結論:左炔諾孕酮引起人子宮肌瘤細胞周期阻滯于G2/M期,并使細胞發(fā)生凋亡,進而抑制其增殖,這可能與該藥物上調P53蛋白的表達,并激活Bcl-2介導的線粒體凋亡途徑有關。
[Abstract]:Aim: to investigate the anti-tumor mechanism of levonorgestrel induced apoptosis in human uterine leiomyoma cells. Methods: the effects of levonorgestrel on the proliferation of human uterine leiomyoma cells and the effects of levonorgestrel on cell cycle and apoptosis of human uterine leiomyoma cells were analyzed by flow cytometry after monolayer culture. The effect of levonorgestrel on p53 apoptosis in human uterine leiomyoma cells was analyzed by Westernblot assay. Results: levonorgestrel significantly inhibited the proliferation of human uterine leiomyoma cells, and levoproterone induced cell cycle arrest in G _ 2 / M phase and induced apoptosis of human uterine leiomyoma cells. Levonorgestrel activates p53 apoptosis pathway in human uterine leiomyoma cells and induces apoptosis. Conclusion: levonorgestrel induces cell cycle arrest in G _ 2 / M phase of human uterine leiomyoma cells and induces cell apoptosis, which may be associated with the up-regulation of p53 protein expression by levonorgestrel. And activation of Bcl-2 mediated mitochondrial apoptosis pathway.
【作者單位】： 中南大學湘雅三醫(yī)院婦產科;
【分類號】：R96
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本文編號：2374361