【摘要】：目的:建立高效液相色譜法測(cè)定普依司他鹽酸鹽原料藥含量。方法:采用Waters Xbridge C18色譜柱(150 mm×4.6 mm,5μm),以0.01 mol·L~(-1)磷酸二氫鈉緩沖液(p H=3.0)-乙腈為流動(dòng)相,梯度洗脫,流速1.0 m L·min~(-1),柱溫30℃,檢測(cè)波長(zhǎng)280 nm。結(jié)果:普依司他鹽酸鹽在6.25~200μg·m L~(-1)范圍內(nèi),峰面積與濃度呈良好線性關(guān)系(R2=0.999 7,n=6)。平均回收率為100.40%(RSD=1.13%,n=9)。結(jié)論:該方法簡(jiǎn)便快速、準(zhǔn)確靈敏、重復(fù)性好,可用于普依司他鹽酸鹽原料藥含量測(cè)定。
[Abstract]:Objective: to establish a HPLC method for the determination of priestat hydrochloride. Methods: Waters Xbridge C18 column (150 mm 脳 4.6 mm,5 渭 m),) was used as mobile phase with 0.01 mol L ~ (-1) sodium dihydrogen phosphate buffer solution (pH 3.0-acetonitrile) as mobile phase, gradient elution, flow rate 1.0 mL min~ (-1). Column temperature 30 鈩，

本文編號(hào)：2354908