液-質(zhì)聯(lián)用法同時測定人血漿伊潘立酮及其兩個代謝產(chǎn)物濃度
發(fā)布時間：2018-11-19 16:43
【摘要】：目的:建立LC-MS/MS法同時測定血漿伊潘立酮(iloperidone,ILP)及其兩個代謝產(chǎn)物羥基伊潘立酮(hydroxy iloperidone,P88)和伊潘立酮羧酸(iloperidone carboxylic acid,P95)的藥濃度。方法:色譜柱為Agilent C8(100 mm×4.6 mm,3.5μm),流動相為乙腈-2 mmol·L-1醋酸銨水溶液(含1.5%甲酸)=28∶72,流速為600μL·min-1,采用ESI源正離子模式,多反應(yīng)監(jiān)測方式測定。ILP,P88,P95的監(jiān)測離子對分別為:m/z 427.2→233.2,m/z 429.2→261.2,m/z 429.2→233.2,內(nèi)標氘代伊潘立酮(iloperidone-D3,ILP-D3)的監(jiān)測離子對為m/z 430.2→233.1。結(jié)果:血漿ILP,P88和P95濃度分別在0.01~15 ng·m L-1,0.01~15ng·m L-1和0.02~20 ng·m L-1范圍內(nèi)線性均良好,日間、日內(nèi)精密度均在可接受范圍內(nèi),提取回收率較高,均大于75%。結(jié)論:該方法準確、靈敏度高、重現(xiàn)性好,能夠較好地應(yīng)用于ILP及其兩個代謝產(chǎn)物的人體藥動學研究。
[Abstract]:Aim: to establish a LC-MS/MS method for the simultaneous determination of plasma epperidone (iloperidone,ILP) and its two metabolites, hydroxy iparidone (hydroxy iloperidone,P88) and epperidone carboxylic acid (iloperidone carboxylic acid,P95). Methods: the chromatographic column consisted of Agilent C8 (100 mm 脳 4.6 mm,3.5 渭 m),) as mobile phase, acetonitrile-2 mmol L-1 ammonium acetate aqueous solution (containing 1.5% formic acid) = 28: 72, and the flow rate of 600 渭 L min-1, was determined by ESI source positive ion mode. The monitoring ion pairs of ILP,P88,P95 are as follows: M / z 427.2 ~ 233.2 m / z 429.2 ~ 261.2 m / z 429.2 ~ (2) N ~ (23 ~ (3.2). The internal standard deuterium dipyridone (iloperidone-D3,) is used as internal standard. The monitored ion pair of ILP-D3 is m / z 430.2 + 233.1. Results: the plasma concentrations of ILP,P88 and P95 were linear in the range of 0.01g / mL ~ (-1) and 0.02o ~ (20) ng 路mL ~ (-1), respectively. The intra-day and intra-day precision were within the acceptable range, and the recovery rate was higher. All are larger than 75. Conclusion: this method is accurate, sensitive and reproducible, and can be applied to the pharmacokinetics of ILP and its two metabolites.
【作者單位】： 蘇州大學醫(yī)學部藥學院;蘇州大學附屬第二醫(yī)院;
【分類號】：R96;O657.63
[Abstract]:Aim: to establish a LC-MS/MS method for the simultaneous determination of plasma epperidone (iloperidone,ILP) and its two metabolites, hydroxy iparidone (hydroxy iloperidone,P88) and epperidone carboxylic acid (iloperidone carboxylic acid,P95). Methods: the chromatographic column consisted of Agilent C8 (100 mm 脳 4.6 mm,3.5 渭 m),) as mobile phase, acetonitrile-2 mmol L-1 ammonium acetate aqueous solution (containing 1.5% formic acid) = 28: 72, and the flow rate of 600 渭 L min-1, was determined by ESI source positive ion mode. The monitoring ion pairs of ILP,P88,P95 are as follows: M / z 427.2 ~ 233.2 m / z 429.2 ~ 261.2 m / z 429.2 ~ (2) N ~ (23 ~ (3.2). The internal standard deuterium dipyridone (iloperidone-D3,) is used as internal standard. The monitored ion pair of ILP-D3 is m / z 430.2 + 233.1. Results: the plasma concentrations of ILP,P88 and P95 were linear in the range of 0.01g / mL ~ (-1) and 0.02o ~ (20) ng 路mL ~ (-1), respectively. The intra-day and intra-day precision were within the acceptable range, and the recovery rate was higher. All are larger than 75. Conclusion: this method is accurate, sensitive and reproducible, and can be applied to the pharmacokinetics of ILP and its two metabolites.
【作者單位】： 蘇州大學醫(yī)學部藥學院;蘇州大學附屬第二醫(yī)院;
【分類號】：R96;O657.63
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