【摘要】：目的制備同時包封IR-780和多柔比星(DOX)的溫敏脂質(zhì)體并進(jìn)行表征。方法采用薄膜水化法及硫酸銨梯度法制備載IR-780和DOX溫敏脂質(zhì)體(DOX-IR-780thermo-sensitive liposome,DITSL)。采用Malvern激光粒度儀檢測各脂質(zhì)體的粒徑、表面電位及多分散系數(shù)(PDI);采用激光誘導(dǎo)DITSL升溫釋藥,檢測各脂質(zhì)體的釋藥特性。結(jié)果 IR-780和DOX同時被包封于溫敏脂質(zhì)體,成功制備得DITSL。IR-780和DOX的包封率分別為(94.47±8.57)%、(92.52±7.61)%;平均粒徑(138.98±8.74)nm;略帶負(fù)電位;PDI為0.32±0.02。激光0.8 W/cm2照射5min,溫度最高升到54.2℃,DOX釋藥率達(dá)80.1%。結(jié)論 DITSL具有藥物包封率較高、粒徑大小適宜、光熱轉(zhuǎn)化效率高、溫度敏感性好的優(yōu)點(diǎn),并可通過激光控制釋藥,為下一步光熱-化療聯(lián)合治療腫瘤的研究奠定了基礎(chǔ)。
[Abstract]:Objective to prepare and characterize the thermo-sensitive liposomes which encapsulate both IR-780 and doxorubicin (DOX) simultaneously. Methods IR-780 and DOX thermo-sensitive liposomes (DOX-IR-780thermo-sensitive liposome,DITSL) were prepared by membrane hydration and ammonium sulfate gradient method. The particle size, surface potential and polydispersity coefficient of liposomes were measured by Malvern laser particle size analyzer. The release characteristics of liposomes were detected by laser induced DITSL. Results the encapsulation efficiency of DITSL.IR-780 and DOX were (94.47 鹵8.57)% and (92.52 鹵7.61)%, respectively, and the mean diameter of DITSL.IR-780 and DOX were (138.98 鹵8.74) nm; with negative potential. PDI was 0.32 鹵0.02. When the laser was irradiated with 0.8 W/cm2 for 5 minutes, the highest temperature was 54.2 鈩，

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