【摘要】：目的建立快速測(cè)定大鼠體內(nèi)西酞普蘭及其代謝物(N-氧化西酞普蘭和N-去甲基西酞普蘭)濃度的超高效液相串聯(lián)質(zhì)譜法。方法用乙酸乙酯萃取法處理血漿,色譜柱為ACQUITY UPLC BEH C18柱(50 mm×2.1 mm,1.7μm);流動(dòng)相為乙腈-0.1%甲酸水,梯度洗脫,流速為0.4 m L·min-1;用正離子多離子反應(yīng)監(jiān)測(cè)(MRM)掃描,內(nèi)標(biāo)為曲馬多。結(jié)果血漿中西酞普蘭、N-去甲基西酞普蘭和N-氧化西酞普蘭的線性范圍分別為0.1~10.0,0.1~10.0,0.01~2.00 ng·m L-1(r=0.999 1,0.999,0.999 6),定量下限分別為0.05,0.05,0.01ng·m L-1。其回收率分別在96.09%~105.33%。三者的日內(nèi)、日間精密度均RSD8.69%。結(jié)論該方法操作簡(jiǎn)便、快捷,靈敏度高,適于大鼠體內(nèi)西酞普蘭及其代謝物的藥代動(dòng)力學(xué)研究。
[Abstract]:Objective to establish a high performance liquid chromatography tandem mass spectrometry method for the rapid determination of citalopram and its metabolites (N-oxide-citalopram and N-demethylcitalopram) in rats. Methods Plasma was treated with ethyl acetate extraction. The chromatographic column was ACQUITY UPLC BEH C18 column (50 mm 脳 2.1 mm,1.7 渭 m); mobile phase was acetonitrile-0.1% formic acid water, gradient elution, flow rate was 0.4 mL min-1;). The (MRM) scan was monitored by positive ion multiion reaction, and the internal standard was tramadol. Results the linear range of plasma citalopram, N-demethylcitalopram and N-oxide-citalopram in plasma was 0.1 ~ 10.0 ~ (10) ~ (10) ~ (-1) ~ (0.1) ~ (-1) ~ 2. 00 ng 路mL ~ (-1) (r ~ (0.999) 1 ~ (0.999) ~ (-1), respectively. The lower limit of quantification was 0.05 ~ 0.05ng / mL ~ (-1), respectively. The recoveries were 96.09 and 105.33, respectively. The intra-day and daytime precision of all three are RSD8.69%.. Conclusion the method is simple, rapid and sensitive. It is suitable for pharmacokinetics of citalopram and its metabolites in rats.
【作者單位】： 麗水市人民醫(yī)院藥學(xué)部;麗水市人民醫(yī)院臨床藥學(xué)實(shí)驗(yàn)室;溫州醫(yī)科大學(xué)藥學(xué)院藥理教研室;
【基金】：衛(wèi)生部行業(yè)科研專(zhuān)項(xiàng)基金資助項(xiàng)目(201302008)
【分類(lèi)號(hào)】：R96;O657.63

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本文編號(hào)：2302301