【摘要】：目的:制備兩性霉素B脂質(zhì)體并對(duì)其藥動(dòng)學(xué)進(jìn)行研究。方法:采用有機(jī)溶劑注入法制備兩性霉素B脂質(zhì)體,用微射流進(jìn)行整粒,用動(dòng)態(tài)光散射法測(cè)定粒徑,采用Ultrafree-CL離心超濾管分離游離兩性霉素B(F-AMB)與復(fù)合兩性霉素B(L-AMB)檢測(cè)包封率,用Wistar大鼠進(jìn)行藥動(dòng)學(xué)研究。結(jié)果:兩性霉素B脂質(zhì)體平均粒徑為(115±20)nm;包封率99.9%;大鼠靜脈注射,實(shí)驗(yàn)藥與Amphotec汶的Cmax,AUC0~168 h和AUC0~∞無(wú)顯著性差異(P0.05)。結(jié)論:采用有機(jī)溶劑注入法制備兩性霉素B脂質(zhì)體具有較高包封率,在大鼠體內(nèi)的藥動(dòng)學(xué)特征與Amphotec汶一致。
[Abstract]:Objective: to prepare amphotericin B liposome and study its pharmacokinetics. Methods: amphotericin B liposomes were prepared by organic solvent injection. The entrapment efficiency of free amphotericin B (F-AMB) and compound amphotericin B (L-AMB) was determined by Ultrafree-CL centrifugation ultrafiltration tube. The pharmacokinetics of Wistar rats was studied. Results: the mean particle size of amphotericin B liposomes was (115 鹵20) nm; entrapment efficiency 99.9, and there was no significant difference in Cmax,AUC0~168 h and AUC0~ 鈭，

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