載納米磺胺嘧啶銀明膠凝膠體外抗菌及體內(nèi)促愈合性能
發(fā)布時(shí)間：2018-10-21 13:44
【摘要】：目的制作包載納米磺胺嘧啶銀(AgSD)的京尼平交聯(lián)明膠凝膠,考察其體外抗菌及體內(nèi)促愈合性能。方法分別配制納米AgSD與粗粉AgSD梯度濃度混懸液、含納米AgSD與粗粉AgSD的明膠交聯(lián)凝膠和不含AgSD的空白凝膠。測(cè)量納米AgSD凝膠對(duì)敏感細(xì)菌的抑菌圈直徑;MTT法考察其對(duì)L929成纖維細(xì)胞的相容性,測(cè)定細(xì)胞相對(duì)生長(zhǎng)率;考察納米AgSD混懸液對(duì)3種敏感細(xì)菌的最小抑菌濃度(MIC)和最小殺菌濃度(MBC);建立小鼠深Ⅱ度燙傷合并金黃色葡萄球菌感染模型,應(yīng)用載藥凝膠敷料治療3周后計(jì)算愈合指數(shù),天狼星紅染色考察小鼠創(chuàng)面膠原沉積,HE染色觀察創(chuàng)面組織病理學(xué)變化并評(píng)分。結(jié)果在抑菌圈、MIC和MBC測(cè)定實(shí)驗(yàn)中,納米AgSD較粗粉混懸液抗菌性能明顯提高,且載納米AgSD凝膠較市售乳膏有更好的抗菌性及細(xì)胞相容性;小鼠創(chuàng)面體內(nèi)愈合實(shí)驗(yàn)中,從給藥第2周開(kāi)始納米AgSD凝膠組創(chuàng)面愈合指數(shù)較涂菌組、乳膏組和粗粉凝膠組顯著提升(P0.05);且與對(duì)照組相比,小鼠創(chuàng)面Ⅰ型膠原、總膠原含量及組織病理學(xué)評(píng)分均明顯升高(P0.05)。結(jié)論載納米AgSD明膠凝膠具有良好的體外抗菌效果及快速促機(jī)體愈合性能。
[Abstract]:Aim to prepare a genipine crosslinked gelatin gel coated with silver sulfadiazine (AgSD) and investigate its antibacterial and healing properties in vitro. Methods the gradient concentration suspensions of nanometer AgSD and crude AgSD, gelatin crosslinked gel containing nanometer AgSD and blank gel without AgSD were prepared respectively. The diameter of bacteriostasis circle of nanometer AgSD gel on sensitive bacteria was measured, the compatibility of L929 fibroblasts and the relative growth rate of L929 fibroblasts were investigated by MTT method. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC);) of nanometer AgSD suspension against three sensitive bacteria were investigated to establish the model of deep second degree scald with Staphylococcus aureus infection in mice. The healing index was calculated after 3 weeks of treatment with drug loaded gel dressing. Sirius red staining was used to study the collagen deposition in the wound and HE staining was used to observe the histopathological changes of the wound. Results in the test of bacteriostatic circle, MIC and MBC, the antibacterial activity of nanometer AgSD was significantly higher than that of crude powder suspension, and the antibacterial and cytocompatibility of nano-AgSD gel was better than that of marketable cream. From the second week after administration, the wound healing index of the AgSD gel group was significantly higher than that of the coated group, the cream group and the crude powder gel group (P0.05), and compared with the control group, the collagen 鈪，
本文編號(hào)：2285253
[Abstract]:Aim to prepare a genipine crosslinked gelatin gel coated with silver sulfadiazine (AgSD) and investigate its antibacterial and healing properties in vitro. Methods the gradient concentration suspensions of nanometer AgSD and crude AgSD, gelatin crosslinked gel containing nanometer AgSD and blank gel without AgSD were prepared respectively. The diameter of bacteriostasis circle of nanometer AgSD gel on sensitive bacteria was measured, the compatibility of L929 fibroblasts and the relative growth rate of L929 fibroblasts were investigated by MTT method. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC);) of nanometer AgSD suspension against three sensitive bacteria were investigated to establish the model of deep second degree scald with Staphylococcus aureus infection in mice. The healing index was calculated after 3 weeks of treatment with drug loaded gel dressing. Sirius red staining was used to study the collagen deposition in the wound and HE staining was used to observe the histopathological changes of the wound. Results in the test of bacteriostatic circle, MIC and MBC, the antibacterial activity of nanometer AgSD was significantly higher than that of crude powder suspension, and the antibacterial and cytocompatibility of nano-AgSD gel was better than that of marketable cream. From the second week after administration, the wound healing index of the AgSD gel group was significantly higher than that of the coated group, the cream group and the crude powder gel group (P0.05), and compared with the control group, the collagen 鈪，
本文編號(hào)：2285253
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