【摘要】：目的： 制備絲素-氧化石墨烯復(fù)合膜(SF-GO),并評價絲素-氧化石墨烯復(fù)合膜的細(xì)胞毒性,為后期在該膜上載藥及動物實驗提供理論依據(jù)。 方法： 1.在普通培養(yǎng)瓶中培養(yǎng)小鼠成纖維細(xì)胞L-929； 2.制備絲素-氧化石墨烯復(fù)合膜； 3.在絲素-氧化石墨烯復(fù)合膜上培養(yǎng)L-929細(xì)胞； 4.采用位相差顯微鏡和電子顯微鏡觀察確認(rèn)L-929細(xì)胞的形態(tài)及其生長情況； 5.采用MTT比色法檢測含有不同濃度氧化石墨烯(0.1wt%.0.2wt%、0.5wt%.1.0wt%)的絲素-氧化石墨烯復(fù)合膜在不同時期(1d、3d、5d、7d)對小鼠成纖維細(xì)胞L929相對增殖率(RGR)的影響,評價其細(xì)胞毒性。 結(jié)果： 隨著氧化石墨烯濃度的增加,細(xì)胞毒性在不斷增加(P0.01)；隨著測定天數(shù)的增加,氧化石墨烯的細(xì)胞毒性減小雖無統(tǒng)計學(xué)意義,但各組總體趨勢都在減��；通過透射電鏡照片可見各組細(xì)胞形態(tài)基本正常,細(xì)胞生長良好,為長梭形或卵圓形,并可見圓形分裂細(xì)胞,細(xì)胞折光性強(qiáng)。 結(jié)論： 氧化石墨烯濃度≤1wt%時無明顯細(xì)胞毒性,可用于后續(xù)進(jìn)一步研究。
[Abstract]:Aim: to prepare fibroin-graphene oxide composite membrane (SF-GO) and evaluate the cytotoxicity of fibroin-graphene oxide composite membrane. Methods: 1. Mouse fibroblasts were cultured in ordinary culture flask. Silk fibroin-graphene oxide composite membrane was prepared. L-929 cells were cultured on fibroin-graphene oxide composite membrane. 4. The morphology and growth of L-929 cells were confirmed by phase contrast microscope and electron microscope. The effects of fibroin-graphene oxide composite membrane containing different concentrations of graphene oxide (0.1 wt.0.2 wtand 0.5 wt.1.0 wt%) on the relative proliferation rate (RGR) of mouse fibroblasts L929 at different periods (1 d ~ 3 d ~ 5 d ~ 7 d) were detected by MTT colorimetry. The cytotoxicity of the membrane was evaluated. Results: with the concentration of graphene oxide increasing, the cytotoxicity of graphene oxide increased (P0.01), the cytotoxicity of graphene oxide decreased with the increase of determination days, but the general trend of each group was decreased. Transmission electron microscopy (TEM) photos showed that the cells in each group were normal in shape, good in growth, long fusiform or oval in shape, round mitotic cells and strong refraction. Conclusion: the concentration of graphene oxide 鈮，

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