【摘要】：目的建立一種高效液相色譜法測(cè)定人全血中羥氯喹的濃度。方法取全血樣本300μL,用3倍量的乙酸乙酯液液提取,以甲硝唑?yàn)閮?nèi)標(biāo),氮?dú)獯蹈珊笥昧鲃?dòng)相60μL復(fù)溶,進(jìn)樣20μL。用Venusil ASB C18(4.6 mm×250 mm,5μm)色譜柱分離,流動(dòng)相為20 mmol·L~(-1)甲酸銨水溶液-乙腈=85∶15(甲酸調(diào)節(jié)流動(dòng)相p H至3.0),流速為0.8 m L·min~(-1),柱溫為35℃,檢測(cè)波長(zhǎng)為254 nm。考察該方法的專(zhuān)屬性,標(biāo)準(zhǔn)曲線和定量下限,精密度和準(zhǔn)確度,穩(wěn)定性和提取回收率。結(jié)果羥氯喹標(biāo)準(zhǔn)曲線方程為f=0.56×10~(-1)C+0.39×10~(-1)(r=0.998 2),在100~3000 ng·m L~(-1)內(nèi)線性關(guān)系良好,日內(nèi)、日間精密度RSD均小于5.5%,全血樣品的提取回收率為(76.08±6.21)%(n=5)。結(jié)論本方法操作簡(jiǎn)潔,靈敏度較高,準(zhǔn)確性好,適用于人全血中羥氯喹濃度的監(jiān)測(cè)。
[Abstract]:Objective to establish a HPLC method for the determination of hydroxychloroquine in human whole blood. Methods 300 渭 L whole blood samples were extracted with 3 times amount of ethyl acetate liquid, metronidazole was used as internal standard, 60 渭 L mobile phase was redissolved in nitrogen, and 20 渭 L was injected. Venusil ASB C18 (4.6 mm 脳 250 mm,5 渭 m) column was used. The mobile phase consisted of 20 mmol L ~ (-1) ammonium formate aqueous solution and acetonitrile = 85:15 (formic acid adjusted mobile phase pH to 3.0), the flow rate was 0.8 mL min~ (-1), the column temperature was 35 鈩，

本文編號(hào)：2270073