【摘要】：目的探討羅格列酮、胰島素干預(yù)下Ⅱ型糖尿病大鼠腦內(nèi)腫瘤壞死因子α誘導(dǎo)蛋白8樣因子2(TIPE2)表達(dá)與細(xì)胞凋亡的變化,以及它們之間的相關(guān)性。方法選取3月齡Wistar大鼠(n=5,3Wistar組)和3月齡GK大鼠(n=5,3GK組);4月齡GK大鼠喂養(yǎng)至6月齡,根據(jù)期間的不同處理分為6月齡GK大鼠(n=5,6GK組)、羅格列酮干預(yù)6月齡GK大鼠(n=5,RSG+6GK組)、胰島素干預(yù)6月齡GK大鼠(n=5,INS+6GK組)。采用免疫組化法檢測(cè)各組大鼠腦內(nèi)TIPE2的表達(dá)部位及特征,運(yùn)用RT-PCR法和Western blotting法檢測(cè)各組大鼠腦內(nèi)TIPE2 mRNA和蛋白的表達(dá),采用免疫熒光法檢測(cè)各組大鼠腦內(nèi)細(xì)胞凋亡的情況。結(jié)果 3GK組在染色強(qiáng)度、陽(yáng)染細(xì)胞數(shù)、陽(yáng)染血管數(shù)、mRNA、蛋白水平均較3 Wistar組有明顯增加(P0.05),6GK組較3GK組有明顯增加(P0.05),RSG+6GK組、INS+6GK組均較6GK組有明顯減少(P0.05)。大鼠腦內(nèi)細(xì)胞凋亡數(shù)目與TIPE2的表達(dá)呈正相關(guān)性(r=0.981 6,P0.05)。結(jié)論Ⅱ型糖尿病可促進(jìn)大鼠腦內(nèi)TIPE2的表達(dá)和細(xì)胞凋亡,隨年齡的增長(zhǎng)和糖尿病病程的進(jìn)展,TIPE2的表達(dá)和細(xì)胞凋亡數(shù)目相應(yīng)增加,羅格列酮、胰島素干預(yù)可減少糖尿病大鼠腦內(nèi)TIPE2的表達(dá)并抑制細(xì)胞凋亡。在糖尿病大鼠腦內(nèi)細(xì)胞凋亡的進(jìn)程中,TIPE2能夠發(fā)揮促凋亡作用。
[Abstract]:Objective to investigate the relationship between the expression of tumor necrosis factor 偽 -inducible protein 8-like factor 2 (TIPE2) and apoptosis in the brain of diabetic rats induced by rosiglitazone and insulin. Methods 3-month-old Wistar rats (n = 5) and 3-month old GK rats (n = 5 GK) were selected, 4 months old GK rats were fed to 6 months old, and 4 months old GK rats were fed to the age of 6 months. According to the different treatments, the rats were divided into 6 month old GK rats (naglitazone group), rosiglitazone group (6 months old GK rats), insulin intervention 6 month old GK rats (nti5ins 6GK group), rosiglitazone treated GK rats at 6 months old (n = 5 RSG 6GK group) and insulin intervention 6 months old GK rats (n = 5 ins 6GK group). Immunohistochemical method was used to detect the expression of TIPE2 in rat brain, RT-PCR and Western blotting methods were used to detect the expression of TIPE2 mRNA and protein in rat brain, and apoptosis was detected by immunofluorescence method. Results the staining intensity, the number of positive staining cells, the number of positive stained blood vessels and the level of mRNA, protein in 3GK group were significantly higher than those in 3 Wistar group (P0.05), and the 6GK group was significantly higher than 3GK group (P0.05), RSG 6GK group, INS 6GK group was significantly lower than 6GK group (P0.05). There was a positive correlation between the number of apoptosis and the expression of TIPE2 in rat brain (r = 0.981 6 P 0.05). Conclusion Type 鈪，

本文編號(hào)：2262218