肉桂醛聯(lián)合兩性霉素B對(duì)煙曲霉菌的體外抗菌活性研究
發(fā)布時(shí)間：2018-10-07 19:56
【摘要】：目的評(píng)價(jià)肉桂醛聯(lián)合兩性霉素B(AMB)應(yīng)用對(duì)煙曲霉菌的體外抗真菌效果,以進(jìn)一步探討聯(lián)合應(yīng)用這兩種藥物能否抑制AMB耐藥煙曲霉菌。方法采用微量液基稀釋法對(duì)臨床分離的30株煙曲霉菌進(jìn)行體外藥敏試驗(yàn),用棋盤(pán)法測(cè)部分抑菌濃度指數(shù)(FIC),評(píng)定兩種藥物聯(lián)合的抗真菌效果。結(jié)果 30株煙曲霉菌中有8株MIC1μg/ml為AMB耐藥菌株,耐藥率為26.7%;肉桂醛與AMB對(duì)30株煙曲霉菌單用時(shí)的MIC50、MIC90及MIC均數(shù)(MICG)比聯(lián)用時(shí)高,差異有統(tǒng)計(jì)學(xué)意義(P0.01);同一藥物的相同濃度下,聯(lián)合應(yīng)用時(shí)的累計(jì)抑菌百分率比單用時(shí)高;肉桂醛與AMB聯(lián)合時(shí)主要表現(xiàn)為協(xié)同、相加作用,部分表現(xiàn)出無(wú)關(guān)作用,未出現(xiàn)拮抗作用。結(jié)論肉桂醛與AMB聯(lián)合應(yīng)用,對(duì)AMB耐藥的煙曲霉菌株主要表現(xiàn)為協(xié)同作用;對(duì)AMB敏感的煙曲霉菌株主要表現(xiàn)為相加作用。
[Abstract]:Objective to evaluate the antifungal effect of cinnamaldehyde combined with amphotericin B (AMB) on Aspergillus fumigatus in vitro, and to explore whether the combined use of these two drugs can inhibit AMB resistant Aspergillus fumigatus. Methods the drug sensitivity of 30 strains of Aspergillus fumigatus was tested by microdilution method in vitro. The antifungal effects of the two drugs were evaluated by chessboard method and partial inhibitory concentration index (FIC),). Results among the 30 strains of Aspergillus fumigatus, 8 strains of MIC1 渭 g/ml were AMB resistant strains, the drug resistance rate was 26.7.The mean number of MIC50,MIC90 and MIC (MICG) of cinnamaldehyde and AMB in 30 Aspergillus fumigatus strains were significantly higher than that of combined use (P0.01), and the same concentration of the same drug had significant difference (P0.01). The cumulative bacteriostatic percentage of combined application was higher than that of single use, and the combination of cinnamaldehyde and AMB was mainly synergistic and additive, and some showed no antagonism. Conclusion the synergistic effect of cinnamaldehyde combined with AMB on AMB resistant aspergillus fumigatus strain and AMB sensitive Aspergillus fumigatus strain is mainly additive.
【作者單位】： 廣西醫(yī)科大學(xué)第一附屬醫(yī)院呼吸疾病研究所;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81260002)
【分類號(hào)】：R96
[Abstract]:Objective to evaluate the antifungal effect of cinnamaldehyde combined with amphotericin B (AMB) on Aspergillus fumigatus in vitro, and to explore whether the combined use of these two drugs can inhibit AMB resistant Aspergillus fumigatus. Methods the drug sensitivity of 30 strains of Aspergillus fumigatus was tested by microdilution method in vitro. The antifungal effects of the two drugs were evaluated by chessboard method and partial inhibitory concentration index (FIC),). Results among the 30 strains of Aspergillus fumigatus, 8 strains of MIC1 渭 g/ml were AMB resistant strains, the drug resistance rate was 26.7.The mean number of MIC50,MIC90 and MIC (MICG) of cinnamaldehyde and AMB in 30 Aspergillus fumigatus strains were significantly higher than that of combined use (P0.01), and the same concentration of the same drug had significant difference (P0.01). The cumulative bacteriostatic percentage of combined application was higher than that of single use, and the combination of cinnamaldehyde and AMB was mainly synergistic and additive, and some showed no antagonism. Conclusion the synergistic effect of cinnamaldehyde combined with AMB on AMB resistant aspergillus fumigatus strain and AMB sensitive Aspergillus fumigatus strain is mainly additive.
【作者單位】： 廣西醫(yī)科大學(xué)第一附屬醫(yī)院呼吸疾病研究所;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81260002)
【分類號(hào)】：R96
【共引文獻(xiàn)】
相關(guān)期刊論文 前1條
1 王潤(rùn)超;萬(wàn)U，
本文編號(hào)：2255479
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