【摘要】：目的:制備聚乙二醇化多西他賽白蛋白納米粒(PEG-albumin nanoparticles,PEG-DANPs),比較市售多西他賽(艾素~?,Aisu~?)、多西他賽白蛋白納米粒(DANPs)和PEG-DANPs三者對裸鼠非小細胞肺癌模型的作用。方法:分別建立BALB/c裸鼠皮下移植瘤及原位癌模型,待皮下組腫瘤體積到達120 mm3時,兩組模型分別通過尾靜脈注射葡萄糖注射液,Aisu~?,DANPs和PEG-DANPs進行治療,每7 d給藥1次,共給藥4次。在最后1次給藥2 d后,將皮下移植瘤組和原位癌組所有裸鼠全部處死,取瘤塊、肺臟、心臟、肝臟、脾送檢HE(hematoxylin-eosin staining)染色切片。另選取裸鼠32只,建立皮下移植瘤模型,分別注射上述相同藥物,持續(xù)治療直至裸鼠自然死亡,最終統(tǒng)計并繪制裸鼠生存曲線。結果:PEG-DANPs能夠有效地抑制裸鼠皮下移植瘤及原位癌的增殖,它能夠有效地控制原位癌的轉移并且延長裸鼠的生存時間。結論:PEGDANPs是一種抗腫瘤細胞效果明顯、不良反應小的藥物,具有廣闊的應用前景。
[Abstract]:Aim: to prepare pegylated docetaxel albumin nanoparticles (PEG-albumin nanoparticles,PEG-DANPs) and compare their effects on nude mice non-small cell lung cancer (NSCLC) models with marketable docetaxel (DANPs), docetaxel albumin nanoparticles (DANPs) and PEG-DANPs. Methods: the models of subcutaneous transplanted tumor and carcinoma in situ were established in nude mice with BALB/c. When the tumor volume of subcutaneous group reached 120 mm3, the two groups were treated by injection of glucose injection (Aisutropium) DANPs and PEG-DANPs respectively every 7 days, for a total of 4 times. After the last administration for 2 days, all the nude mice in the subcutaneous transplanted tumor group and the carcinoma in situ group were killed and HE (hematoxylin-eosin staining) staining sections were taken from the tumor mass, lung, heart, liver and spleen. Another 32 nude mice were selected to establish the tumor model of subcutaneous transplantation. The same drug was injected into the nude mice. The treatment lasted until the nude mice died naturally. Finally, the survival curve of the nude mice was calculated and drawn. Results the cell proliferation of xenografted tumor and carcinoma in situ was inhibited effectively by 1% PEG-DANPs. It could effectively control the metastasis of carcinoma in situ and prolong the survival time of nude mice. ConclusionWith PEGDANPs is an effective antitumor drug with little adverse reaction, and has a broad application prospect.
【作者單位】： 延邊大學附屬醫(yī)院;中國醫(yī)學科學院藥物研究所天然藥物活性物質與功能國家重點實驗室藥物傳輸技術及新型制劑北京市重點實驗室;
【分類號】：R-332;R965

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