【摘要】：目的研究沙利度胺對雙膦酸鹽相關(guān)頜骨壞死的影響。方法 36只大鼠隨機(jī)分成A、B、C組,分別接受生理鹽水、唑來膦酸、唑來膦酸+沙利度胺治療。用藥3周后,拔除大鼠左上頜第1磨牙。拔牙后4、8周,收獲標(biāo)本,評價(jià)頜骨壞死、微血管生成及細(xì)胞凋亡情況。結(jié)果拔牙后4、8周,大鼠上頜骨拔牙創(chuàng)處均無死骨裸露,但B組、C組部分標(biāo)本可見一些小的瘺管。組織學(xué)檢查顯示,A組標(biāo)本未見死骨,而B組、C組在拔牙窩周圍可見小塊死骨。B組、C組拔牙窩周圍區(qū)域空骨陷窩百分比和死骨面積顯著多于A組(P0.01),而骨陷窩密度則顯著下降。B組、C組微血管密度較A組也顯著降低,在4周時(shí)分別下降了和25.87%和55.27%(P0.01),在8周時(shí)分別下降了45.62%和72.84%(P0.01);凋亡細(xì)胞數(shù)則在4周時(shí)分別增長了54.80%和87.89%(P0.01),在8周分別增長了208.08%和250.58%(P0.01)。結(jié)論沙利度胺加重了唑來膦酸誘發(fā)的早期階段的頜骨壞死;沙利度胺和唑來膦酸對頜骨壞死的作用與微血管生成抑制有關(guān)。
[Abstract]:Objective to study the effect of thalidomide on bisphosphonate-associated osteonecrosis of jaws. Methods Thirty-six rats were randomly divided into two groups: group A, B, C, treated with normal saline and thalidomide zoledronate, respectively. After 3 weeks, the left maxillary first molar was extracted. Eight weeks after tooth extraction, specimens were harvested to evaluate jaw necrosis, microangiogenesis and apoptosis. Results No dead bone was exposed at the site of maxillary extraction in rats 4 weeks after tooth extraction, but some small fistula was found in some specimens of group B and C. Histological examination showed that no dead bone was found in group A. The percentage of empty bone lacunae and dead bone area in group C were significantly higher than those in group A (P0.01), while the density of bone lacunae in group C was significantly lower than that in group A (P0.01). The microvessel density of group C was significantly lower than that of group A (P 0.01). The number of apoptotic cells decreased by 54.80% and 87.89% (P0.01) at 4 weeks, and by 208.08% and 250.58% (P0.01) at 8 weeks, respectively, and decreased by 45.62% and 72.84% (P0.01) at week 4 and by 25.87% and 55.27% (P0.01) respectively at week 4, and by 45.62% and 72.84% (P0.01) at week 8, respectively, and the number of apoptotic cells increased by 54.80% and 87.89% (P0.01) respectively. Conclusion thalidomide exacerbates the early stage of jaw necrosis induced by zoledronic acid, and the effects of thalidomide and zoledronic acid on jaw necrosis are related to the inhibition of microangiogenesis.
【作者單位】： 華北理工大學(xué)口腔醫(yī)學(xué)院口腔頜面外科;華北理工大學(xué)基礎(chǔ)醫(yī)學(xué)院病理教研室;
【基金】：國家自然科學(xué)基金(81270965,81101448) 河北省自然科學(xué)基金(C2011401044)~~
【分類號】：R965

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 彭誠;王東;;雙膦酸鹽類藥物治療相關(guān)的下頜骨壞死的研究進(jìn)展[J];醫(yī)學(xué)綜述;2011年08期

2 黃如冰;吳曉林;俞娟;;雙磷酸鹽相關(guān)性頜骨壞死12例臨床分析[J];中華口腔醫(yī)學(xué)研究雜志(電子版);2012年04期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 黃金玲;王靚;王桐生;桑方方;侯曉燕;劉先華;;化痰通瘀解毒方對S180小鼠移植瘤MVD及VEGF表達(dá)的影響[J];中醫(yī)藥臨床雜志;2011年03期

2 羅永紅;何愛保;張軼清;;基質(zhì)金屬蛋白酶-2及其組織抑制因子表達(dá)失衡與卵巢上皮性腫瘤血管生成的關(guān)系[J];安徽醫(yī)學(xué);2007年02期

3 金東華;葉學(xué)忠;;NF-κBp65在肝癌血管生成中的作用[J];安徽醫(yī)學(xué);2009年03期

4 吳丹;孫國平;彭光輝;馬泰;仲飛;;胃癌組織中HER-2表達(dá)及與血管生成的關(guān)系[J];安徽醫(yī)藥;2012年03期

5 夏仲元;李曰慶;錢麗旗;潘琳;李鴻;;疏肝通絡(luò)方對大鼠乳腺增生血管生成的影響[J];安徽中醫(yī)學(xué)院學(xué)報(bào);2010年03期

6 劉曼曼;余濤;陳e，

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