【摘要】：目的:制備出一種具有生物可降解性的搭載克林霉素的聚乳酸-羥基乙酸共聚物(PLGA)緩釋微球,并對其相關(guān)性質(zhì)進(jìn)行研究。方法:采用乳化-溶劑揮發(fā)法制備搭載克林霉素的PLGA微球,檢測克林霉素-PLGA微球表面形貌和粒徑分布,觀察PLGA/二氯甲烷(P/D)與PLGA/克林霉素(P/C)不同投藥比對微球包封率的影響;用適量的釋放介質(zhì)分散緩釋微球后,37℃震蕩,取各時(shí)間點(diǎn)的藥液,采用K-B紙片瓊脂擴(kuò)散法檢測不同時(shí)間點(diǎn)的抑菌環(huán)直徑大小評價(jià)微球體外緩釋抑菌能力。結(jié)果:微球的形體圓整、表面光滑、無明顯黏連,且粒徑大小主要集中在700nm左右。當(dāng)投藥比為P/D=80/1、P/C=5/1時(shí)包封率最佳,為45.02%,包覆效果良好。微球在第1~2天時(shí)體外抑菌效果最為明顯,第3~19天呈現(xiàn)持續(xù)穩(wěn)定的抑菌效果,在第20天時(shí)降至藥物的最小抑菌濃度(MIC)。結(jié)論:克林霉素-PLGA微球的制備工藝良好,其體外抑菌效果表現(xiàn)出明顯的緩釋性。
[Abstract]:Aim: to prepare a biodegradable poly (lactic acid-glycolic acid) (PLGA) sustained-release microspheres with clindamycin. Methods: PLGA microspheres loaded with clindamycin were prepared by emulsification-solvent volatilization method. The surface morphology and particle size distribution of clindamycin PLGA microspheres were measured. The effects of different dosages of PLGA/ dichloromethane (P / D) and PLGA/ clindamycin (P / C) on the encapsulation efficiency of the microspheres were observed. The sustained release microspheres were oscillated at 37 鈩，

本文編號(hào)：2233401