【摘要】：目的建立一種利用細(xì)胞電阻抗傳感技術(shù)(ECIS)進(jìn)行體外藥物篩選與評(píng)價(jià)的方法。方法利用MTT法測(cè)定NCI-H460細(xì)胞對(duì)紫杉醇注射液、多西他賽注射液、長(zhǎng)春瑞濱注射液、注射用培美曲塞二鈉及順鉑注射液的敏感性,再分別采用ECIS和MTT法測(cè)定紫杉醇、多西他賽、長(zhǎng)春瑞濱和培美曲塞二鈉4種抗腫瘤藥對(duì)NCI-H460細(xì)胞生長(zhǎng)的影響。借助配對(duì)t檢驗(yàn)、組內(nèi)相關(guān)系數(shù)以及Bland-Altman法等對(duì)兩種方法所測(cè)得抑制率進(jìn)行一致性綜合評(píng)價(jià),結(jié)合電子顯微鏡下細(xì)胞的形態(tài)學(xué)觀察,評(píng)估所建立的方法。結(jié)果通過(guò)MTT法測(cè)得NCI-H460細(xì)胞對(duì)紫杉醇及多西他賽注射液敏感,對(duì)長(zhǎng)春瑞濱注射液及注射用培美曲塞二鈉呈中度敏感,順鉑對(duì)NCI-H460細(xì)胞株的抑制率無(wú)明顯變化。紫杉醇、多西他賽、長(zhǎng)春瑞濱、培美曲塞二鈉4種不同藥物對(duì)NCI-H460細(xì)胞作用的動(dòng)態(tài)過(guò)程可通過(guò)ECIS動(dòng)態(tài)監(jiān)測(cè)法進(jìn)行定性定量表征,同時(shí)還可反映出不同藥物作用的特征性差異。另外,通過(guò)分析抑制率數(shù)據(jù)結(jié)果,顯示出兩種方法一致性較好,結(jié)合電子顯微鏡觀察不同時(shí)期NCI-H460細(xì)胞形態(tài)更進(jìn)一步說(shuō)明此法的可行性。結(jié)論所建立的基于細(xì)胞電阻抗分析方法可用于體外藥物篩選與評(píng)價(jià),可有效地表征藥物體外抗腫瘤活性,在腫瘤耐藥性及個(gè)體化給藥研究方面具有廣闊的臨床應(yīng)用前景。
[Abstract]:Objective to establish a method for drug screening and evaluation in vitro by cell impedance sensing technique (ECIS). Methods the sensitivity of NCI-H460 cells to paclitaxel injection, docetaxel injection, vinorelbine injection, pemetrexed disodium injection and cisplatin injection was determined by MTT assay. Paclitaxel and docetaxel were determined by ECIS and MTT respectively. Effects of vinorelbine and pemetrexide on the growth of NCI-H460 cells. By means of paired t test, intra-group correlation coefficient and Bland-Altman method, the consistency of the inhibition rate measured by the two methods was evaluated, and the established method was evaluated with the morphological observation of the cells under electron microscope. Results MTT assay showed that NCI-H460 cells were sensitive to paclitaxel and docetaxel injection, moderately sensitive to vinorelbine injection and pemetrexide disodium for injection, and the inhibition rate of cisplatin on NCI-H460 cell line was not significantly changed. The dynamic process of paclitaxel, docetaxel, vinorelbine and pemetrexed disodium on NCI-H460 cells can be qualitatively and quantitatively characterized by ECIS dynamic monitoring method, and also can reflect the characteristic difference of different drugs. In addition, by analyzing the inhibition rate data, the results show that the two methods are in good agreement, combined with the electron microscope to observe the morphology of NCI-H460 cells at different stages to further illustrate the feasibility of this method. Conclusion the method based on cell impedance analysis can be used to screen and evaluate drugs in vitro, and can effectively characterize the antitumor activity of drugs in vitro. It has a broad clinical application prospect in the research of tumor resistance and individualized administration.
【作者單位】： 軍事醫(yī)學(xué)科學(xué)院毒物藥物研究所;軍事醫(yī)學(xué)科學(xué)院附屬醫(yī)院藥學(xué)部;
【基金】：軍事醫(yī)學(xué)科學(xué)院軍事醫(yī)學(xué)創(chuàng)新基金資助項(xiàng)目(2012CXJJ017)
【分類號(hào)】：R96
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本文編號(hào)：2230795