【摘要】：為了探討殼寡糖(COS)對(duì)He La細(xì)胞和A549細(xì)胞自噬性死亡的影響,利用不同濃度COS處理細(xì)胞后MTT法檢測(cè)細(xì)胞的增殖抑制情況,采用MDC染色法觀察自噬泡的形成,免疫熒光法檢測(cè)LC3在細(xì)胞內(nèi)的表達(dá),Western-blot分析COS處理He La和A549細(xì)胞不同時(shí)間后細(xì)胞內(nèi)LC3-Ⅱ/LC3-Ⅰ變化。結(jié)果表明,5.0 mg·m L-1COS對(duì)兩種細(xì)胞的增殖抑制率最大,分別為52.15%和57.63%(P0.01)。與陰性對(duì)照相比,經(jīng)COS處理后的細(xì)胞MDC染色細(xì)胞內(nèi)熒光強(qiáng)度增加,且陽(yáng)性信號(hào)聚集于核周區(qū)域;免疫熒光檢測(cè)發(fā)現(xiàn)LC3陽(yáng)性顆粒數(shù)明顯增加,表明細(xì)胞內(nèi)自噬體的數(shù)量增多。Western-blot檢測(cè)分析隨著COS處理細(xì)胞時(shí)間延長(zhǎng),LC3-Ⅱ/LC3-Ⅰ比值逐漸增大,顯示細(xì)胞內(nèi)自噬活性增強(qiáng)。由此推測(cè)COS能夠通過(guò)誘導(dǎo)He La和A549細(xì)胞的自噬性死亡來(lái)抑制細(xì)胞增殖,為COS的抗腫瘤作用機(jī)制的研究提供理論依據(jù)。
[Abstract]:In order to investigate the effect of chitosan oligosaccharide (COS) on autophagy death of He La cells and A549 cells, the inhibition of cell proliferation was detected by MTT method after treated with different concentrations of COS, and the formation of autophagy was observed by MDC staining. The expression of LC3 in He La and A549 cells was detected by Western blot with immunofluorescence assay. The changes of LC3- 鈪，

本文編號(hào)：2227809