【摘要】：目的通過代謝組學(xué)方法研究卡托普利干預(yù)自發(fā)性高血壓大鼠血清內(nèi)源性代謝物的變化,進(jìn)一步探索卡托普利的作用機(jī)制。方法采用快速高分離液相-四級(jí)桿飛行時(shí)間串聯(lián)質(zhì)譜聯(lián)用技術(shù)采集大鼠血清內(nèi)源性代謝物信息,經(jīng)偏最小二乘法判別分析,識(shí)別顯著差異的變量,鑒定潛在生物標(biāo)志物。結(jié)果正常組、模型組、卡托普利組的血清內(nèi)源性代謝物、代謝模式發(fā)生了明顯變化。經(jīng)數(shù)據(jù)庫查詢共確定了4個(gè)生物標(biāo)記物及其代謝途徑,與血管內(nèi)皮功能密切相關(guān)。結(jié)論代謝組學(xué)從機(jī)體整體代謝角度揭示了卡托普利保護(hù)血管內(nèi)皮功能的可能作用機(jī)制,在闡釋藥物復(fù)雜的作用機(jī)制方面顯示出了獨(dú)特的潛力。
[Abstract]:Objective to investigate the effects of captopril on the changes of serum endogenous metabolites in spontaneously hypertensive rats and to explore the mechanism of captopril. Methods the information of endogenous metabolites in rat serum was collected by high separation liquid-four-pole time-of-flight tandem mass spectrometry. By partial least squares discriminant analysis, significant differences were identified and potential biomarkers were identified. Results the serum endogenous metabolites and metabolic patterns of normal group, model group and captopril group were obviously changed. Four biomarkers and their metabolic pathways were identified by database query, which were closely related to vascular endothelial function. Conclusion Metabonomics reveals the possible mechanism of captopril in protecting vascular endothelial function from the point of view of whole body metabolism and shows unique potential in explaining the complex mechanism of drugs.
【作者單位】： 山東中醫(yī)藥大學(xué)第一臨床醫(yī)學(xué)院;山東中醫(yī)藥大學(xué)實(shí)驗(yàn)中心;山東中醫(yī)藥大學(xué)附屬醫(yī)院高血壓國家中藥臨床研究基地;
【基金】：中國博士后科學(xué)基金特別資助項(xiàng)目(No 2015T80741) 國家自然科學(xué)基金資助項(xiàng)目(No 81473653)
【分類號(hào)】：R965

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