【摘要】：目的:考察醋酸曲安奈德微透析探針體外回收率的影響因素,探索提高微透析探針回收率的方法。方法:采用高效液相色譜法考察溫度、灌流液流速、探針類型以及灌流液成分(羥丙基-β-環(huán)糊精、脂肪乳、牛血清白蛋白)對醋酸曲安奈德體外回收率的影響。結(jié)果:醋酸曲安耐德探針回收率隨溫度增高而增加;隨灌流液流速增加而降低;不同類型的探針對醋酸曲安耐德回收率不同;灌流液成分會對醋酸曲安奈德回收率產(chǎn)生較大影響。結(jié)論:采用PBS(p H 7.4)溶液添加1%或2%牛血清白蛋白為灌流液,醋酸曲安奈德體外探針回收率得到較大提高,且回收率和傳遞率相等。
[Abstract]:Aim: to investigate the factors influencing the in vitro recovery of triamcinolone acetonide acetate microdialysis probe and to explore the method to improve the recovery rate of microdialysis probe. Methods: the effects of temperature, flow rate, probe type and composition (hydroxypropyl- 尾 -cyclodextrin, fat emulsion, bovine serum albumin) on the in vitro recovery of triamcinolone acetonide acetate were investigated by HPLC. Results: the recovery rate of triamide acetate probe increased with the increase of temperature and decreased with the increase of flow rate of perfusion fluid. The composition of perfusate has a great effect on the recovery of triamcinolone acetonide acetate. Conclusion: the in vitro probe recovery of triamcinolone acetonide acetate was improved by adding 1% or 2% bovine serum albumin into PBS (p H 7.4) solution, and the recovery and transfer rate were equal.
【作者單位】： 中國醫(yī)學(xué)科學(xué)院皮膚病研究所;
【基金】：中央級公益性科研院所基本科研業(yè)務(wù)專項(xiàng)基金(YZ-08-01) 楊森科學(xué)研究委員會中國分會研究基金(JRCC2012皮科01)
【分類號】：R927
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本文編號：2199681