【摘要】：目的:建立同時測定氯霉素地砜軟膏中氯霉素、氨苯砜及地塞米松含量的方法。方法:采用反相高效液相色譜法。色譜柱為Agilent ZORBAX SB-C18,流動相為0.1%庚烷磺酸鈉溶液-甲醇(70∶30,V/V),流速為1.0 ml/min,檢測波長為272 nm,柱溫為30℃,進樣量為20μl。結(jié)果:氯霉素、氨苯砜和地塞米松的質(zhì)量濃度分別在20.12~201.2、10.25~102.5、0.202 6~2.026μg/ml范圍內(nèi)與各自峰面積呈良好線性關系(r=0.998 7、0.999 9、0.999 9);精密度、重復性、穩(wěn)定性試驗的RSD≤1.19%;回收率分別為99.66%(RSD=0.34%,n=9)、99.84%(RSD=0.41%,n=9)與99.94%(RSD=0.49%,n=9)。結(jié)論:該方法操作簡便快捷,可為氯霉素地砜軟膏的質(zhì)量控制提供參考。
[Abstract]:Objective: to establish a method for simultaneous determination of chloramphenicol, dapsone and dexamethasone in chloramphenicol dissulfone ointment. Methods: RP-HPLC was used. The chromatographic column was Agilent ZORBAX SB-C18, the mobile phase was 0.1% sodium heptanesulfonate-methanol (70: 30 V / V), the flow rate was 1.0 ml / min, the detection wavelength was 272nm, the column temperature was 30 鈩，

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