發(fā)布時間：2018-08-07 22:00

【摘要】：目的:建立同時測定人工牛黃甲硝唑膠囊中來源于原料人工牛黃中的5種膽汁酸類成分(�；悄懰徕c、膽酸、豬去氧膽酸、鵝去氧膽酸和去氧膽酸)的方法。方法:采用高效液相色譜儀與蒸發(fā)光散射檢測器聯(lián)用技術(shù)對人工牛黃甲硝唑膠囊進(jìn)行測定。色譜條件:Agilent TC-C18色譜柱(4.6 mm×250 mm,5μm),柱溫35℃;流動相為0.2%甲酸水溶液(A)-乙腈(B),梯度洗脫(0~10 min,15%B→32%B;10~15 min,32%B→50%B;15~22 min,50%B→68%B;22~23 min,68%B→95%B;23~28 min,95%B),流速1.0 m L·min-1;ELSD檢測器:氮氣流速2.3 L·min-1,漂移管溫度115℃。結(jié)果:�；悄懰徕c、膽酸、豬去氧膽酸、鵝去氧膽酸、去氧膽酸質(zhì)量濃度分別在24.93~249.27、47.62~476.19、39.72-397.23、6.29~62.92和10.63~106.28μg·m L-1范圍內(nèi)與峰面積成良好對數(shù)線性關(guān)系;平均回收率(n=6)分別為99.9%(RSD=1.6%)、98.9%(RSD=1.6%)、99.2%(RSD=2.0%)、99.4%(RSD=2.0%)和98.6%(RSD=2.0%);3批樣品測定結(jié)果分別為0.16%~0.37%、0.67%~0.75%、0.44%~0.70%、0.10%~0.17%、0.16%~0.19%。結(jié)論:該法可同時測定人工牛黃甲硝唑膠囊中來源于原料人工牛黃的5種膽汁酸類成分(�；悄懰徕c、膽酸、豬去氧膽酸、鵝去氧膽酸和去氧膽酸)的含量,適用于人工牛黃甲硝唑膠囊的質(zhì)量評價與控制。
[Abstract]:Objective: to establish a method for simultaneous determination of five kinds of bile acids (sodium taurocholate, cholic acid, porcine deoxycholic acid, chenodeoxycholic acid and deoxycholic acid) in Metronidazole capsules. Methods: the method of high performance liquid chromatograph and evaporative light scattering detector was used to determine the artificial bezoar metronidazole capsules. The mobile phase consisted of 0.2% formic acid aqueous solution (A) and acetonitrile (B), gradient elution (0.10 min ~ 15B ~ 15B ~ 1015 min) with a column temperature of 4.6mm 脳 250mm ~ 5 渭 m),. The mobile phase consisted of a flow rate of 1.0 mL 路min -1 ELSD detector: nitrogen flow rate 2.3 L -1, drift tube temperature 115 鈩，

本文編號：2171475