【摘要】：目的:研究真皮內(nèi)注射二甲氨基乙醇(DMAE)復(fù)合制劑對(duì)亞急性衰老大鼠皮膚膠原代謝及TGFβ1/smads通道的影響。方法:30只Wistar大鼠隨機(jī)分入衰老生理鹽水組(A組)、衰老治療組(B組)、正常對(duì)照組(C組),各10只。A、B兩組頸背部皮下注射D-半乳糖125mg/kg/d 42天。從第18天開始于A、B組臀背部分別注射生理鹽水和DMAE復(fù)合制劑1ml,每周一次,連續(xù)四周。每次治療后3天進(jìn)行活體皮膚共聚焦纖維鏡檢測(cè)。42天處死所有大鼠,取注射藥物區(qū)域皮膚,觀察各組皮膚真皮厚度、膠原纖維密度和皮膚羥脯氨酸(HYP)含量的變化,RT-PCR法檢測(cè)皮膚組織TGFβ1、smad3、Ⅰ、Ⅲ型前膠原(colⅠcolⅢ)脂質(zhì)金屬蛋白酶-1(MMP-1)、脂質(zhì)金屬蛋白酶抑制劑-1(TIMP-1)m RNA表達(dá)量,以及免疫組化檢測(cè)成纖維細(xì)胞增殖細(xì)胞核抗原(PCNA)和TGFβ1的表達(dá)。結(jié)果:衰老治療組與衰老生理鹽水組大鼠相比真皮厚度、膠原纖維密度、羥脯氨酸含量及TIMP-1 m RNA表達(dá)量顯著增加,皮膚組織colⅠ、TGFβ1、smad3 m RNA表達(dá)量增加(P0.05),而對(duì)colⅢ、MMP-1 m RNA及PCNA表達(dá)量沒有影響。結(jié)論:真皮內(nèi)注射DMAE復(fù)合制劑(賽雷弗#174;)可正調(diào)節(jié)衰老皮膚中成纖維細(xì)胞TGFβ1/smad3基因表達(dá),促進(jìn)Ⅰ型膠原蛋白合成,增加衰老皮膚中膠原的含量和真皮厚度,且通過(guò)調(diào)節(jié)MMP-1/TIMP-1平衡抑制膠原蛋白降解發(fā)揮其抗衰老作用。
[Abstract]:Aim: to study the effects of intradermal injection of dimethylamino ethanol (DMAE) compound preparation on collagen metabolism and TGF- 尾 1/smads channel in subacute aging rats. Methods Thirty Wistar rats were randomly divided into aging saline group (group A), aging treatment group (group B) and normal control group (group C). 10 rats in each group were subcutaneously injected with D-galactose 125mg/kg/d for 42 days. From the 18th day, 1 ml of saline and DMAE were injected into the buttock back of group A and B respectively, once a week for four weeks. Three days after each treatment, all rats were killed by confocal fibroscope examination of living skin on day 42, and the dermal thickness of each group was observed by injecting drug area skin. The changes of collagen fiber density and hydroxyproline (HYP) content in skin were used to detect the mRNA expression of col 鈪，

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