【摘要】：目的:研究沒食子酸與氟康唑聯(lián)用對耐藥光滑念珠菌和克柔念珠菌的體外抗真菌活性。方法:采用棋盤微量稀釋法測定沒食子酸與氟康唑單用及聯(lián)用對耐藥光滑念珠菌和克柔念珠菌的抗真菌作用,以部分抑菌濃度指數(shù)(FICI)評價其聯(lián)用效果;并以CG2、CK8為目標菌株,采用時間-殺菌曲線進行驗證;通過測定沒食子酸對耐藥株生物膜形成早期黏附性的影響,初步探討藥物聯(lián)合抗真菌耐藥的作用。結(jié)果:聯(lián)用時8~16μg·m L-1沒食子酸能將氟康唑的MIC值降為單用時的1/4~1/8,FICI值為0.375,呈現(xiàn)較好的協(xié)同作用,且量效關(guān)系呈正相關(guān)。時間-殺菌曲線表明,與氟康唑單用相比,沒食子酸與氟康唑聯(lián)用24 h時,CG2、CK8菌株CFU的lg值分別下降了2.1個單位和2.25個單位,均表現(xiàn)為協(xié)同作用。另外,研究發(fā)現(xiàn)沒食子酸對生物膜形成的早期黏附有明顯的抑制作用。結(jié)論:沒食子酸與氟康唑聯(lián)用對耐藥光滑念珠菌和克柔念珠菌的浮游菌表現(xiàn)出體外協(xié)同抗真菌作用,且沒食子酸能夠明顯抑制非白色念珠菌生物膜形成的早期黏附。
[Abstract]:Aim: to study the in vitro antifungal activity of Gallic acid combined with fluconazole against resistant Candida glabra and Candida krrolimus in vitro. Methods: the antifungal effects of Gallic acid and fluconazole on Candida smooth and Candida krsurolimus were determined by chessboard microdilution method, and the combined effect was evaluated by partial inhibitory concentration index (FICI). The effects of Gallic acid on the early adhesion of drug-resistant biofilm were determined by time-sterilization curve, and the effect of combination of drug and antifungal resistance on the formation of biofilm was preliminarily investigated. Results: Gallic acid could reduce the MIC value of fluconazole to 1 / 4 / 8 / 8 FICI of 1 / 4 / 1 / 8, which showed a good synergistic effect, and the dose-effect relationship was positively correlated with the combined use of Gallic acid at 8 ~ 16 渭 g / m ~ (-1), and the MIC value of fluconazole was reduced to 1 / 4 / 8 / 8 FICI of 1 / 4 / 8. The time-bactericidal curve showed that compared with fluconazole alone, the LG value of CFU decreased by 2.1 units and 2.25 units respectively when Gallic acid and fluconazole were combined with fluconazole for 24 h, showing synergistic effect. In addition, it was found that Gallic acid could inhibit the early adhesion of biofilm. Conclusion: the combination of Gallic acid and fluconazole showed synergistic antifungal effect on resistant Candida glabra and Candida krrolimus in vitro, and Gallic acid could significantly inhibit the early adhesion of non-white candida biofilm formation.
【作者單位】： 山東省泰山醫(yī)院;山東大學(xué)附屬千佛山醫(yī)院;
【基金】：山東省科技發(fā)展計劃課題(編號:2013GSF11848)
【分類號】：R96
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本文編號：2129787