本文選題：過氧化氫 + 氧化應(yīng)激��； 參考：《吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2015年03期

【摘要】：目的:利用過氧化氫(H2O2)構(gòu)建人宮頸癌Hela細(xì)胞體外氧化應(yīng)激模型,觀察其對Hela細(xì)胞自噬和凋亡的影響,并探討H2O2與內(nèi)質(zhì)網(wǎng)應(yīng)激(ERS)信號通路之間的關(guān)系。方法:以1mmol·L-1 H2O2構(gòu)建體外氧化應(yīng)激模型;實(shí)驗(yàn)分為對照組、H2O24h組、H2O28h組和H2O212h組,各組細(xì)胞分別以H2O2作用0、4、8及12h后,利用Western blotting法檢測各組Hela細(xì)胞中ERS及凋亡、自噬相關(guān)蛋白表達(dá)。結(jié)果:與對照組比較,各H2O2組Hela細(xì)胞中Cleaved Caspase-3表達(dá)水平升高(P0.05),Cleaved Caspase-4表達(dá)水平亦明顯升高(P0.05);各H2O2組Hela細(xì)胞中LC3-Ⅱ表達(dá)水平高于對照組(P0.05),GRP78表達(dá)水平亦高于對照組(P0.05);隨著H2O2作用時(shí)間的延長,各H2O2組Hela細(xì)胞中IRE1a表達(dá)水平明顯升高(P0.01),p-JNK表達(dá)水平明顯高于對照組(P0.05)。結(jié)論:在H2O2所構(gòu)建的體外氧化應(yīng)激模型中H2O2能夠誘導(dǎo)Hela細(xì)胞發(fā)生自噬和凋亡,且該誘導(dǎo)作用由ERS信號通路介導(dǎo)。
[Abstract]:Aim: to investigate the effects of hydrogen peroxide (H _ 2O _ 2) on autophagy and apoptosis of human cervical cancer Hela cells in vitro, and to explore the relationship between H2O2 and endoplasmic reticulum stress (ERS) signaling pathway. Methods: 1mmol L-1 H 2O 2 was used to construct oxidative stress model in vitro, and the cells were divided into two groups: control group (H 2O 2 24 h), H 2O 2 group (H 2O 2 28 h) and H 2O 2 group (H 2O 2 12 h). After treated with H 2O 2 for 0 h and 12 h respectively, the expression of ERS, apoptosis and autophagy related protein in Hela cells were detected by Western blotting assay. Results: compared with the control group, The expression of Cleaved Caspase-3 in Hela cells increased significantly (P0.05), the expression of LC3- 鈪，

本文編號：2111175