發(fā)布時間：2018-06-21 15:50

  本文選題：高鐵血紅蛋白 + 快速��； 參考：《重慶醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的： 明確不同濃度亞硝酸鈉溶液與高鐵血紅蛋白生成率間的量效關(guān)系方程，采用RGB色彩模式，研制出一種基于比色卡原理技術(shù)的、快速、準(zhǔn)確、安全且經(jīng)濟實用的人血高鐵血紅蛋白濃度檢測卡。 方法： 利用不同濃度亞硝酸鈉溶液孵育健康成人獻(xiàn)血者血樣，得到亞硝酸鈉溶液與高鐵血紅蛋白生成率間的量效關(guān)系方程。根據(jù)亞硝酸鈉溶液與高鐵血紅蛋白生成率間的量效關(guān)系方程，獲取濃度百分比為10%～80%（間隔10%）高鐵血紅蛋白血樣所需的不同濃度亞硝酸鈉溶液。 將健康獻(xiàn)血者未經(jīng)孵育血樣與濃度百分比為10%～80%（間隔10%）高鐵血紅蛋白血樣分別滴加在血樣采集卡上，并用RGB ColorFinder軟件掃描獲取紅綠藍(lán)（red, green, blue, RGB）值和顏色十六進(jìn)制代碼（HEX Code），最終生成相應(yīng)的高鐵血紅蛋白色塊，制成檢測卡。 結(jié)果： 亞硝酸鈉溶液（X）與高鐵血紅蛋白生成率（Y）間的量效關(guān)系方程為：Y=2.12598+0.7123X（R2=0.9982，P0.01），，亞硝酸鈉溶液與高鐵血紅蛋白生成率間呈線性相關(guān)。當(dāng)需形成濃度百分比為10%～80%（間隔10%）高鐵血紅蛋白時，亞硝酸鈉溶液濃度分別為11mmol/L、25mmol/L、39mmol/L、53mmol/L、67mmol/L、81mmol/L、95mmol/L、109mmol/L。 根據(jù)健康成人獻(xiàn)血者血樣及濃度百分比為10%～80%（間隔10%）高鐵血紅蛋白血樣獲取的RGB值分別為：（205.10.16）、（193.33.35）、（183.34.38）、（170.35.39）、（157.36.41）、（144.37.43）、（133.38.44）、（119.39.48）、(103.40.49)；HEX Code值為（#CD0A10）、（#C12123）、（#B7226）、（#AA2327）、（#9D2429）、（#90252B）、（#85262C）、（#772730）、（#672831）。與健康成人獻(xiàn)血者血樣相比，隨著高鐵血紅蛋白濃度百分比的增高，血樣采集卡上的顏色逐漸變深至棕褐色。 結(jié)論： 本實驗所制作而成的人血高鐵血紅蛋白濃度快速檢測卡，為法醫(yī)日常檢案、刑事偵查及臨床醫(yī)務(wù)工作者檢測人血高鐵血紅蛋白的濃度提供了快速、準(zhǔn)確、安全且經(jīng)濟實用的檢測方法，值得推廣使用。
[Abstract]:Objective: to determine the dose-effect relation equation between sodium nitrite solution and methemoglobin production rate in different concentrations, and to develop a rapid and accurate method based on the principle of colorimetric card by using RGB color model. Safe and economical human blood methemoglobin concentration test card. Methods: blood samples of healthy adult blood donors were incubated with different concentrations of sodium nitrite solution, and the dose-effect relationship equation between sodium nitrite solution and methemoglobin production rate was obtained. According to the dose-effect relation equation between sodium nitrite solution and methemoglobin production rate, the different concentrations of sodium nitrite solution needed for the blood sample of methemoglobin were obtained. Add the unincubated blood samples and the blood samples of the healthy donors to the blood sample collection card with a percentage of 10% and 80% (interval 10) of methemoglobin, The RGB ColorFinder software was used to scan red, green, blue, RGBand hexadecimal codes of HEX code. Finally, the corresponding methemoglobin color blocks were generated, and the detection cards were made. Results: the equation of dose-effect relationship between sodium nitrite solution (X) and methemoglobin production rate (Y) was: 1: Y2.12598 0.7123XC R2O0.9982P0.01C, and there was a linear correlation between sodium nitrite solution and methemoglobin production rate. When methemoglobin needs to be formed in a percentage of 10 mmol / L, the concentration of sodium nitrite solution is 11 mmol / L / L ~ (25) mmol / L ~ (39) mmol / L ~ (53) mmol / L ~ (87) mmol / L ~ (95) mmol / L ~ (10) mmol / L ~ (109) mmol 路L ~ (-1). 鏍規(guī)嵁鍋ュ悍鎴愪漢鐚鑰呰鏍峰強嫻撳害鐧懼垎姣斾負(fù)10%锝

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