本文選題：殼聚糖 + 殼寡糖��； 參考：《藥物分析雜志》2017年11期

【摘要】：目的:為了快速準(zhǔn)確的進(jìn)行殼寡糖樣品的檢測,建立一種分離度好、靈敏度高的殼寡糖離子色譜快速檢測方法。方法:實驗選用Dionex-Carbo Pac PA104×50 mm離子交換色譜柱,通過優(yōu)化柱溫、淋洗液配比及濃度等色譜條件,將不同聚合度的殼寡糖在30 min內(nèi)實現(xiàn)分離。分離后的殼寡糖樣品通過計算聚合度為殼2~6糖的含量,檢驗其樣品中殼寡糖的純度。結(jié)果:樣品濃度在2.00~10.00μg·m L~(-1)范圍內(nèi)線性關(guān)系良好(r≥0.999 0),精密度實驗RSD在0.1%~1.6%之間,樣品加標(biāo)回收率93.2%~105.0%。3種殼寡糖樣品中2~6個聚合度殼寡糖的含量之和分別為25.944μg·g~(-1)、26.406μg·g~(-1)、25.374μg·g~(-1),計算得到純度為86.5%、88.0%和84.6%,符合國家標(biāo)準(zhǔn)要求。結(jié)論:該方法需要樣品量少,檢驗快速,無需衍生,為殼寡糖質(zhì)量分析、組分分離提供了依據(jù)。
[Abstract]:Objective: to establish a rapid and sensitive method for the determination of chitosan oligosaccharides by ion chromatography. Methods: Dionex-Carbo PA104 脳 50mm ion exchange column was used to separate chitosan oligosaccharides with different degree of polymerization within 30 min by optimizing the column temperature, eluant ratio and concentration. The purity of chitosan oligosaccharide was tested by calculating the degree of polymerization of chitosan oligosaccharide to be the content of chitosan oligosaccharide. Results: the linear range of sample concentration was 2.00 渭 g / L ~ (-1), r 鈮，

本文編號：2048843