本文選題：血液 + 血小板　； 參考：《中國輸血雜志》2017年10期

【摘要】：目的探討濃縮血小板保存期內(nèi)不同時間制備的去白細(xì)胞混合濃縮血小板制劑的質(zhì)量。方法采用2種時間點制備去白細(xì)胞混合濃縮血小板:方法 1為常規(guī)制備方法,將采血后24 h內(nèi)經(jīng)白膜法制備的5袋同型濃縮血小板匯集后去除白細(xì)胞,并在血小板保存箱內(nèi)振蕩保存7 d;方法 2為改良法,將采血后24 h內(nèi)經(jīng)白膜法常規(guī)制備的濃縮血小板在血小板保存箱內(nèi)振蕩保存,d5將同型5袋濃縮血小板匯集,用一次性白細(xì)胞輸血過濾器過濾處理,制備成混合濃縮血小板制劑,再在血小板保存箱內(nèi)保存2 d。2種方式制備的混合濃縮血小板制劑各15袋,檢測保存0d,5 d和7 d時血小板含量、紅細(xì)胞混入量、血小板代謝情況及炎性因子含量等質(zhì)量指標(biāo)。結(jié)果 2種方法制備的產(chǎn)品質(zhì)量在常規(guī)的保存5 d均可達(dá)到國家標(biāo)準(zhǔn)。改良法血小板保存d7與常規(guī)法d7比較,細(xì)胞因子IL-6(84.80±13.45vs 3.83±0.44)pg/m L,IL-10(67.90±12.02 vs 14.93±2.98)pg/m L,TNF-!(43.17±5.12 vs 7.58±0.71)pg/m L明顯升高,差異具統(tǒng)計學(xué)意義(P值0.01),其它指標(biāo)沒有明顯差異。結(jié)論濃縮血小板在制備后5 d內(nèi)均可再進(jìn)行匯集成混合濃縮血小板制劑。
[Abstract]:Objective to study the quality of leukocyte-free mixed concentrated platelet preparation prepared at different times during the storage period of concentrated platelets. Methods two different time points were used to prepare leukocyte-free mixed concentrated platelets: method 1: 5 bags of homotypic concentrated platelets prepared by white membrane method within 24 hours after blood collection were collected and leukocytes were removed. Methods the concentrated platelets prepared by the white membrane method within 24 hours after the blood collection were stored in the platelet storage box for 7 days, and the same type 5 bags of concentrated platelets were collected in the platelet storage box for 5 days. A mixed concentrated platelet preparation was prepared by filter treatment with a single leukocyte transfusion filter, and 15 bags of each of the mixed concentrated platelet preparations were prepared in the platelet storage box for 2 days. The content of platelet, the amount of red blood cell mixed in, the metabolism of platelet and the content of inflammatory factor were measured at 5 and 7 days after preservation. Results the quality of the two methods could reach the national standard after 5 days of preservation. Compared with the routine method, the cytokines IL-64.80 鹵13.45vs 3.83 鹵0.44)pg/m IL-107.90 鹵12.02 vs 14.93 鹵2.98)pg/m / L TNF-N 43.17 鹵5.12 vs 7.58 鹵0.71)pg/m L were significantly higher than those of the conventional method (P < 0.01). Conclusion the concentrated platelets can be assembled into a mixed concentrated platelet preparation within 5 days after preparation.
【作者單位】： 涪陵區(qū)中心血站;涪陵中心醫(yī)院;
【基金】：科研課題:混合濃縮血小板的制備及臨床應(yīng)用研究(FLKJ,2015AABB1095)
【分類號】：R943
，

本文編號：2027311