本文選題：芹菜素 + 雄性大鼠��； 參考：《蘭州大學(xué)》2014年碩士論文

【摘要】：目的通過芹菜素灌胃,觀察芹菜素在不同劑量、不同時(shí)間對雄性昆明種小鼠和SD大鼠的血液生化指標(biāo)、肝臟氧化還原指標(biāo)和血液氧化還原指標(biāo)的影響,探討其劑量效應(yīng),為芹菜素的深入研究和進(jìn)一步的開發(fā)利用提供科學(xué)依據(jù)。 方法1.將200只健康SPF級雄性昆明種小鼠按體重隨機(jī)分為4組,分別為陰性對照組(生理鹽水10ml/kg)、低劑量組(AP252mg/kg)、中劑量組(AP504mg/kg)和高劑量組(AP1008mg/kg),每組50只。每天灌胃1次,每周6次,連續(xù)灌胃5周。在實(shí)驗(yàn)第7天、14天、21天、28天、35天,分批眼眶內(nèi)眥靜脈叢取血,檢測血清總蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)、丙氨酸氨基轉(zhuǎn)移酶(ALT)、天冬氨酸氨基轉(zhuǎn)移酶(AST)、谷氨酰轉(zhuǎn)肽酶(GGT)和堿性磷酸酶(ALP)活力。頸椎脫臼處死小鼠后取肝臟,制備10%肝臟組織勻漿。比色法檢測肝臟組織勻漿中總抗氧化能力(T-AOC)、還原型谷胱甘肽/氧化型谷胱甘肽(GSH/GSSG)、丙二醛(MDA)、超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、谷胱甘肽過氧化物酶(GSH-Px)吸光值,并計(jì)算各指標(biāo)酶活力。 2.將48只健康SPF級雄性SD大鼠按體重隨機(jī)分為4組,分別為對照組(生理鹽水10ml/kg)、低劑量組(AP234mg/kg)、中劑量組(AP468mg/kg)和高劑量組(AP936mg/kg),每組12只。每天灌胃1次,每周6次,連續(xù)灌胃5周。實(shí)驗(yàn)第35天心臟穿刺取血5ml,檢測血清總蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)、丙氨酸氨基轉(zhuǎn)移酶(ALT)、天冬氨酸氨基轉(zhuǎn)移酶(AST)活力和血清丙二醛(MDA)、超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、總抗氧化能力(T-AOC)、谷胱甘肽過氧化物酶(GSH-Px)、谷胱甘肽(GSH)含量。處死大鼠,取出肝臟后-20℃保存待用。檢測前制備10%肝臟組織勻漿,檢測丙二醛(MDA)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、總抗氧化能力(T-AOC)、谷胱甘肽過氧化物酶(GSH-Px)、谷胱甘肽(GSH)含量,并計(jì)算各指標(biāo)酶活力。在光鏡下觀察大鼠肝臟組織的病理學(xué)改變。 結(jié)果1.體重小鼠體重14天中劑量組(31.27±3.32g)與對照組(35.53±2.84g)比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；大鼠體重呈現(xiàn)增長趨勢,各劑量組與對照組比較差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。 2.臟器系數(shù)小鼠肝臟系數(shù)14天中劑量組(3.96±0.44)×10-2與對照組(4.42±0.63)×10-2比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；28天低劑量組(4.23±0.39)×10-2、中劑量組(3.88±0.40)×10-2、高劑量組(4.15±0.41)×10-2與對照組(4.57±0.38)×10-2比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；35天高劑量組(4.15±0.39)×10-2與對照組(4.47±0.28)X10-2比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。大鼠肝臟重量、脾臟重量,肝/體比(%)、脾/體比(‰),與各自對照組比較差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。 3.血清生化小鼠7天高劑量組血清TP、ALB、ALT、AST、ALP與相應(yīng)對照組比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)14天中劑量組血清中TP、 ALB、ALT、AST與相應(yīng)對照組比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；21天低劑量組血清中TP、ALB、ALT、AST與相應(yīng)對照組比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；35天ALB、ALT、AST和ALP低劑量組、中劑量組、高劑量組與對照組比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。大鼠血清TP、ALB、 GLO低、中、高劑量組與對照組比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。ALT、 AST低、中、高劑量組與各自對照組比較差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。 4.大鼠血清氧化還原酶大鼠血清SOD低劑量組(178.43±7.22U/mg)與對照組(159.07±22.07U/mg)比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；CAT高劑量組(9.45±3.90U/m1)與對照組(5.31±1.79U/m1)比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05); GSH-Px低劑量組(1922.09±180.38U/mg)與對照組(2154.22±173.41U/mg)比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；T-AOC低(9.00±3.08U/mg)、中(7.85±2.40U/mg)、高劑量組(9.59±2.32U/mg)與對照組(12.03±2.82U/mg)比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 5.小鼠肝臟氧化還原酶小鼠肝臟T-AOC在7天(1.68±0.82U/mmg)、14天(1.04±0.42U/mmg)、28天(1.08±0.45U/mg)高劑量組與對照組比較均降低,且差異有統(tǒng)計(jì)學(xué)意義(P0.05)；21天中劑量組(2.04±1.05)與對照組(1.43±1.58)比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05); GSH/GSSG在28天低劑量組(1.70±1.61)與對照組(1.70±1.61)比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；MDA在21天高劑量組(1.07±0.73nmol/mg)與對照組(0.35±0.21nmol/mg)比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；SOD在28天高劑量組(100.73±10.87U/mg)與對照組(111.60±3.36U/mg)比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；35天中劑量組(91.30±13.99U/mg)與對照組(108.00±6.04U/mg)比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05); CAT在7天高劑量組(50.60±28.06U/m1)與對照組(22.70±11.15U/m1)比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；35天高劑量組(115.19±36.43U/ml)與對照組(69.84±32.10U/ml)比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05):GSH-Px在21天低劑量組(70.21±40.61U/mg)與對照組(40.07±9.18U/mg)比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；28天高劑量組(33.43±17.77U/mg)與對照組(50.85±13.12U/mg)比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 6.大鼠肝臟氧化還原酶大鼠肝臟MDA低(0.66±0.13nmol/mg).中(0.31±0.05nmol/mg).高(0.40±0.07nmol/mg)劑量組與對照組(0.48±0.06nmol/mg)比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)；NOS低劑量組(0.74±0.19U/ml)與對照組(0.49±0.17U/ml)比較升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；SOD中(19.59±3.16U/mg)、高(19.48±1.91U/mg)劑量組與對照組(24.81±2.72U/mg)比較均降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05);CAT低(53.37±8.89U/ml)、中(36.48±3.79U/ml)、高(42.97±6.11U/ml)劑量組與對照組(68.66±6.50U/ml)比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05);T-AOC低(0.44±0.02U/mg)、中(0.44±0.09U/mg)、高(0.37±0.08U/mg)劑量組與對照組(0.74±0.12U/mg)比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)；GSH-Px低(290.37±57.71U/mg)、中(346.79±41.75U/mg)高(243.18±42.89U/mg)劑量組與對照組(403.66±92.83U/mg)比較降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論芹菜素亞慢性暴露可影響雄性小鼠和大鼠的肝臟功能；芹菜素在不同劑量、不同時(shí)間對不同品系動物在不同組織表現(xiàn)出的抗/促氧化作用并不一致,提示黃酮類化合物在不同劑量下表現(xiàn)出的作用是不同的,作為膳食抗氧化劑應(yīng)用時(shí),應(yīng)該有一個(gè)適宜的攝入時(shí)間和劑量。
[Abstract]:Objective To observe the effect of apigenin on the blood biochemical index, liver redox index and blood oxidation-reduction index of male Kunming mice and SD rats in different doses and time, and to provide a scientific basis for the in-depth study and further development of apigenin in different doses and time.
Method 1. 200 healthy SPF male Kunming mice were randomly divided into 4 groups according to their weight. They were negative control group (10ml/kg), low dose group (AP252mg/kg), medium dose group (AP504mg/kg) and high dose group (AP1008mg/kg), 50 rats in each group, 1 times a day, 6 times a week for 5 weeks. The experiment was seventh days, 21 days, 28 days, 35 days, Serum total protein (TP), albumin (ALB), globulin (GLO), alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutamine transaminopeptidase (GGT) and alkaline phosphatase (ALP) activity were detected in the orbital canthus plexus, and 10% liver tissue homogenate was prepared after the dehydration of the cervical vertebra in mice. The liver tissue was detected by colorimetric assay. The total antioxidant capacity (T-AOC) in the homogenate, reduced glutathione / oxidized glutathione (GSH/GSSG), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and calculated the activity of each index.
2. 48 healthy SPF grade male SD rats were randomly divided into 4 groups according to their weight. They were the control group (physiological saline 10ml/kg), low dose group (AP234mg/kg), middle dose group (AP468mg/kg) and high dose group (AP936mg/kg), each group was filled with 1 times a day, 6 times a week for 5 weeks, and thirty-fifth days of heart puncture, blood 5ml, and serum total protein (TP) were detected. Albumin (ALB), globulin (GLO), alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity and serum malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), glutathione (GSH) content. 10% liver tissue homogenate was prepared before detection, and the content of malondialdehyde (MDA), nitric oxide synthase (NOS), superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), glutathione (GSH), and the enzyme activity were calculated. The pathological changes of liver tissues of rats were observed under light microscope. Change.
Results the weight of 1. weight mice in 14 days (31.27 + 3.32g) was lower than that of the control group (35.53 + 2.84g). The difference was statistically significant (P0.05), and the weight of the rats showed a trend of growth, and there was no significant difference between the different dose groups and the control group (P0.05).
The liver coefficient of the 2. organ coefficient mice was 14 days (3.96 + 0.44) x 10-2 and the control group (4.42 + 0.63) x 10-2. The difference was statistically significant (P0.05); the low dose group for 28 days (4.23 + 0.39) x 10-2, the middle dose group (3.88 +) * * *, the high dose group was compared with the control group. The difference was statistically significant. The study significance (P0.05); the 35 day high dose group (4.15 + 0.39) x 10-2 and the control group (4.47 + 0.28) X10-2 decreased, the difference was statistically significant (P0.05). The liver weight, spleen weight, liver / body ratio (%), spleen / body ratio (%), and the comparison of the control group were not statistically significant (P0.05).
3. the serum TP, ALB, ALT, AST, ALP in the high dose group of serum biochemical mice were higher than those in the corresponding control group (P0.05), the difference was statistically significant (P0.05) in the serum of 14 days, TP, ALB, ALT, AST were higher than the corresponding control group, and the difference was statistically significant (P0.05), and the low dose group was higher than the corresponding control group at the low dose of 21 days. The difference was statistically significant (P0.05), 35 days ALB, ALT, AST and ALP low dose group, middle dose group, the high dose group was lower than the control group, the difference was statistically significant (P0.05). The serum TP, ALB, GLO low, the high dose group was lower than the control group, the difference was statistically significant (P0.05).ALT, low AST, high dose group and their control groups The difference was not statistically significant (P0.05).
The serum SOD low dose group (178.43 + 7.22U/mg) and the control group (159.07 + 22.07U/mg) were higher in the 4. rats. The difference was statistically significant (P0.05); the high dose group of CAT (9.45 + 3.90U/m1) was higher than the control group (5.31 + 1.79U/m1), and the difference was statistically significant (P0.05); the low dose group of GSH-Px (1922.09 + 180.38U/mg) was compared with the control group (5.31 + 1.79U/m1). The control group (2154.22 + 173.41U/mg) was lower, the difference was statistically significant (P0.05); T-AOC was low (9 + 3.08U/mg), middle (7.85 + 2.40U/mg), high dose group (9.59 + 2.32U/mg) and control group (12.03 + 2.82U/mg) decreased, the difference was statistically significant (P0.05).
5. the liver T-AOC of mice liver oxidoreductase was 7 days (1.68 + 0.82U/mmg), 14 days (1.04 + 0.42U/mmg), 28 days (1.08 + 0.45U/mg) in high dose group decreased with the control group, and the difference was statistically significant (P0.05). In 21 days, the dose group (2.04 + 1.05) was significantly higher than that in the group (1.43 + 1.58), and the difference was statistically significant (P0.05); GSH/GSSG In the 28 day low dose group (1.70 + 1.61) and the control group (1.70 + 1.61), the difference was statistically significant (P0.05); MDA was higher in the 21 day high dose group (1.07 + 0.73nmol/mg) and the control group (0.35 + 0.21nmol/mg), and the difference was statistically significant (P0.05); SOD in the high dose group 28 days (100.73 + 10.87U/mg) and the control group (111.60 + 3.36U/mg) ratio. The difference was statistically significant (P0.05); in the 35 day medium dose group (91.30 + 13.99U/mg) and the control group (108 + 6.04U/mg), the difference was statistically significant (P0.05); CAT was higher in the 7 day high dose group (50.60 + 28.06U/m1) and the control group (22.70 + 11.15U/m1), the difference was statistically significant (P0.05), and the 35 day high dose group (115.19 + 3). 6.43U/ml) compared with the control group (69.84 + 32.10U/ml), the difference was statistically significant (P0.05): GSH-Px was higher in the 21 day low dose group (70.21 + 40.61U/mg) and the control group (40.07 + 9.18U/mg), and the difference was statistically significant (P0.05); the 28 day high dose group (33.43 + 17.77U/mg) was lower than the control group (50.85 + 13.12U/mg), and the difference was statistically significant. Learning meaning (P0.05).
The liver MDA of the 6. rat liver oxidoreductase was low (0.66 + 0.13nmol/mg). (0.31 + 0.13nmol/mg). The high (0.40 + 0.07nmol/mg) dose group was significantly different from the control group (0.48 + 0.06nmol/mg), and the NOS low dose group (0.74 + 0.19U/ml) was higher than that of the group (0.49 + 0.17U/ml), and the difference was statistically significant (P0.05); S OD (19.59 + 3.16U/mg), high (19.48 + 1.91U/mg) dose group and control group (24.81 + 2.72U/mg) were lower, the difference was statistically significant (P0.05), CAT low (53.37 + 8.89U/ml), (36.48 + 3.79U/ml), high (42.97 + 6.11U/ml) dose group and the control group (68.66 + 6.50U/ml) decreased, the difference was statistically significant (P0.05); low T-AOC (0.44 + 42.97). G), in the medium (0.44 + 0.09U/mg), the high (0.37 + 0.08U/mg) dose group was lower than the control group (0.74 + 0.12U/mg), the difference was statistically significant (P0.05), the GSH-Px low (290.37 + 57.71U/mg), the middle (346.79 + 41.75U/mg) high (243.18 + 42.89U/mg) dose group were lower than the control group (403.66 + 92.83U/mg), and the difference was statistically significant (P0.05).
Conclusion apigenin subchronic exposure can affect the liver function of male and female mice. Apigenin has different anti / anti oxidative effects on different tissues at different doses and different time, suggesting that the effects of flavonoids in different doses are different and are used as dietary antioxidants. There should be a suitable intake time and dose.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R965

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉茂如;鎘生產(chǎn)時(shí)混合性粉塵致病作用的評價(jià)[J];中國職業(yè)醫(yī)學(xué);1982年05期

2 王|蘭;芳香族烴類經(jīng)皮膚的滲透量[J];國外醫(yī)學(xué).衛(wèi)生學(xué)分冊;1982年01期

3 張寅恭;新殺蟲劑Пиримор的衛(wèi)生毒理學(xué)[J];國外醫(yī)學(xué).衛(wèi)生學(xué)分冊;1982年01期

4 蔡海英,張金生,崔小邢,鄭國墚,黃孔威;國產(chǎn)維甲酸衍生物對大鼠食管上皮癌變的抑制作用[J];癌癥;1983年04期

5 高強(qiáng);大鼠直腸給心得安以避免首過消除效應(yīng)[J];國外醫(yī)學(xué).藥學(xué)分冊;1983年01期

6 Seigo SHUMIYA;Sumi NAGASE;蔡華琬;;大鼠(Rattus Norvegicus)無白蛋白基因(Aualbumia)和蓋帽基因(Hooded)的連鎖[J];實(shí)驗(yàn)動物與比較醫(yī)學(xué);1983年01期

7 陳國志,黃淑英,王廣義,劉志紅;豚鼠和大鼠小腸移行性肌電復(fù)合波(MMC)的觀察[J];基礎(chǔ)醫(yī)學(xué)與臨床;1984年03期

8 橫路謙次郎;不同類型輻射照射復(fù)合催乳素誘發(fā)大鼠乳腺腫瘤的效果[J];輻射防護(hù);1984年03期

9 溫志永;;幼鼠夜間PR 峰的出現(xiàn)對孕酮的依賴作用[J];國外醫(yī)學(xué).婦產(chǎn)科學(xué)分冊;1989年05期

10 趙學(xué)增;鄭富穩(wěn);夏燁;;普萘洛爾對雌性大鼠生殖機(jī)能的影響[J];河北醫(yī)科大學(xué)學(xué)報(bào);1989年03期

相關(guān)會議論文 前10條

1 程井軍;吳其愷;彭銳;孫國杰;;電針對非酒精性脂肪性肝炎大鼠肝組織氧化及抗氧化狀態(tài)的影響[A];中華中醫(yī)藥學(xué)會第十三屆內(nèi)科肝膽病學(xué)術(shù)會議論文匯編[C];2008年

2 楊s，

本文編號：2015192