本文選題：卡維地洛 + 人肝星狀細(xì)胞�。� 參考：《中國(guó)藥房》2017年19期

【摘要】：目的:研究卡維地洛對(duì)瘦素誘導(dǎo)的LX2人肝星狀細(xì)胞(HSC-LX2)活化增殖的影響及機(jī)制。方法:取對(duì)數(shù)生長(zhǎng)期的HSC-LX2細(xì)胞分為空白對(duì)照組、瘦素刺激組和卡維地洛低、中、高濃度組(5、10、20μmol/L),除空白對(duì)照組外,其余各組均加入0.1 g/L的瘦素及相應(yīng)濃度的卡維地洛作用24 h。采用MTT法檢測(cè)細(xì)胞的光密度(OD)值,計(jì)算細(xì)胞增殖抑制率;流式細(xì)胞術(shù)檢測(cè)細(xì)胞周期和凋亡情況;實(shí)時(shí)熒光定量聚合酶鏈?zhǔn)椒磻?yīng)法檢測(cè)細(xì)胞中α-平滑肌肌動(dòng)蛋白(α-SMA)、基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)、瘦素及瘦素受體m RNA表達(dá);Western blot法檢測(cè)磷酸化Janus激酶2(p-JAK2)、磷酸化信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子3(p-STAT3)蛋白表達(dá)。結(jié)果:與空白對(duì)照組比較,瘦素刺激組細(xì)胞的OD值增加、凋亡率降低、G_0/G_1期細(xì)胞減少(P0.05);α-SMA、TIMP-1、瘦素、瘦素受體m RNA表達(dá)和p-JAK2、p-STAT3蛋白表達(dá)均增強(qiáng)(P0.05)。與瘦素刺激組比較,卡維地洛各濃度組細(xì)胞的OD值減小、凋亡率升高、細(xì)胞主要阻滯在G_0/G_1期(P0.05);α-SMA、TIMP-1、瘦素、瘦素受體m RNA表達(dá)和p-JAK2、p-STAT3蛋白表達(dá)均減弱(P0.05),且呈濃度依賴性(P0.05)。結(jié)論:卡維地洛能夠抑制瘦素誘導(dǎo)的HSC-LX2細(xì)胞的活化增殖,促進(jìn)HSC-LX2細(xì)胞凋亡;其機(jī)制可能與下調(diào)瘦素、瘦素受體基因表達(dá)并阻斷瘦素誘導(dǎo)的細(xì)胞內(nèi)JAK2/STAT3信號(hào)通路激活有關(guān)。
[Abstract]:Objective: To study the effect and mechanism of carvedilol on the activation and proliferation of LX2 human hepatic stellate cells (HSC-LX2) induced by leptin. Methods: the HSC-LX2 cells from the logarithmic growth period were divided into blank control group, leptin stimulation group and carvedilol low, medium and high concentration group (5,10,20 mu mol/L), except for the blank control group, all the other groups were added to 0.1 g/L leptin and phase. The concentration of carvedilol at 24 h. was used to detect cell light density (OD) and cell proliferation inhibition rate; cell cycle and apoptosis were measured by flow cytometry; alpha smooth muscle actin (alpha -SMA), matrix metalloproteinase inhibitor 1 (TIMP-1), leptin and leptin were detected by real-time fluorescence quantitative polymerase chain reaction. The expression of leptin receptor m RNA; Western blot method to detect the phosphorylated Janus kinase 2 (p-JAK2), phosphorylation signal transduction and transcription activator 3 (p-STAT3) protein expression. Results: compared with the blank control group, the OD value of the cells in the leptin stimulation group increased, the apoptosis rate decreased and the G_0/G_1 phase cell decreased (P0.05); alpha -SMA, TIMP-1, leptin, leptin receptor M And p-JAK2, the expression of p-STAT3 protein increased (P0.05). Compared with the leptin stimulation group, the OD value of cells in the concentration group of carvilol decreased, the apoptosis rate increased, and the cells mainly blocked in the G_0/G_1 phase (P0.05); alpha -SMA, TIMP-1, leptin, m RNA expression of M, p-JAK2, p-STAT3 protein decreased (P0.05), and showed concentration dependence. Carvedilol can inhibit the activation and proliferation of leptin induced HSC-LX2 cells and promote apoptosis of HSC-LX2 cells. The mechanism may be related to the downregulation of leptin, leptin receptor gene expression and blocking the activation of JAK2/STAT3 signaling pathway induced by leptin.
【作者單位】： 河北省人民醫(yī)院門(mén)診辦公室;河北省人民醫(yī)院代謝病重點(diǎn)實(shí)驗(yàn)點(diǎn);河北省人民醫(yī)院神經(jīng)外二科;河北省人民醫(yī)院消化內(nèi)二科;
【基金】：河北省醫(yī)學(xué)科學(xué)研究重點(diǎn)課題計(jì)劃項(xiàng)目(No.ZL20140250)
【分類號(hào)】：R96

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 吳劍鋒;徐迪暉;趙海金;;高糖對(duì)人肝星狀細(xì)胞的激活作用及機(jī)制[J];成都醫(yī)學(xué)院學(xué)報(bào);2013年06期

2 張玉婷;張騫騫;劉立新;;內(nèi)源性轉(zhuǎn)化生長(zhǎng)因子β1對(duì)人肝星狀細(xì)胞胰島素樣生長(zhǎng)因子結(jié)合蛋白相關(guān)蛋白1表達(dá)的影響[J];中華消化病與影像雜志(電子版);2012年03期

3 秦h覬，

本文編號(hào)：1980964