發(fā)布時間：2018-05-28 20:14

  本文選題：黃芪總苷 + MK-��； 參考：《中國醫(yī)院藥學(xué)雜志》2017年18期

【摘要】：目的:研究黃芪總苷和地卓西平馬來酸(MK-801)對宮內(nèi)窘迫后的缺氧后新生鼠的Tau蛋白磷酸化程度的影響。方法:采用2×3析因設(shè)計:即宮內(nèi)窘迫(2水平:無處置、施行宮內(nèi)窘迫即夾閉孕鼠的子宮動脈2/3持續(xù)10 min)和藥物(3水平:生理鹽水、MK-801、黃芪總苷)的所有組合。新生鼠生長至12周時留取海馬,高效液相色譜(HPLC)檢測谷氨酸(Glu)含量,IHC-SP檢測p-AT8Ser202與GSK-3β1H8表達。結(jié)果:黃芪總苷可降低宮內(nèi)窘迫誘發(fā)的Glu濃度升高;MK-801對海馬中Glu的濃度無明顯影響。宮內(nèi)窘迫可增加海馬內(nèi)磷酸化程度的Tau蛋白p-AT8Ser202以及促進Tau蛋白磷酸化程度的調(diào)控蛋白GSK-3β1H8的表達;黃芪總苷和MK-801可減緩p-AT8~(Ser202)磷酸化程度。結(jié)論:黃芪總苷可以通過降低海馬Glu濃度減緩Tau蛋白的磷酸化;GSK-3β是該信號通路的關(guān)鍵蛋白;黃芪總苷改善由于宮內(nèi)窘迫誘發(fā)導(dǎo)致缺氧后的學(xué)習(xí)記憶能力低下的效果優(yōu)于MK-801。
[Abstract]:Aim: to study the effects of total astragalus glycosides and didroxepine maleate (MK-801) on the phosphorylation of Tau protein in neonatal rats after hypoxia. Methods: the 2 脳 3 factorial design was used: the level of intrauterine distress 2: no treatment, the uterine artery of pregnant rats was clipped by intrauterine distress for 2 / 3 for 10 min) and the level of drug 3: normal saline MK-801, total astragaloside). The hippocampus was taken from newborn rats at 12 weeks of growth, and the content of glutamate was detected by HPLC. IHC-SP was used to detect the expression of p-AT8Ser202 and GSK-3 尾 1H8. Results: total astragalus glycosides could decrease the concentration of Glu induced by intrauterine distress. MK-801 had no effect on the concentration of Glu in hippocampus. Intrauterine distress increased the expression of Tau protein p-AT8Ser202 in hippocampus and GSK-3 尾 1H8, which promoted the phosphorylation of Tau protein, and the total astragaloside and MK-801 decreased the phosphorylation of p-AT8 / Ser202. Conclusion: total astragalus glycosides can slow down the phosphorylation of Tau protein by decreasing the concentration of Glu in hippocampus and GSK-3 尾 is the key protein in this signaling pathway, and the effect of Astragalus glycoside on learning and memory impairment after hypoxia induced by intrauterine distress is better than that of MK-801.
【作者單位】： 棗莊市立醫(yī)院藥學(xué)部;
【分類號】：R965

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