本文選題：黑根霉 + 胞外多糖　； 參考：《天然產(chǎn)物研究與開發(fā)》2017年10期

【摘要】：采用單因素實驗結(jié)合Box-Behnken中心組合設(shè)計方法,以蛋白質(zhì)脫除率和綜合指數(shù)為指標(biāo),考察木瓜蛋白酶濃度、酶解溫度和酶解時間對黑根霉胞外多糖酶-Sevag法脫蛋白工藝的影響。經(jīng)過響應(yīng)面實驗優(yōu)化,確定最優(yōu)條件為木瓜蛋白酶濃度425 U/mL、酶解溫度62℃、酶解時間90 min,經(jīng)三次重復(fù)實驗驗證,蛋白質(zhì)脫除率與綜合指數(shù)均值分別為54.08%和2.07。
[Abstract]:The effects of papain concentration, enzymatic hydrolysis temperature and enzymatic hydrolysis time on the deproteinization of Rhizopus nigra by extracellular polysaccharide enzyme -Sevag method were investigated by single factor experiment and Box-Behnken center combination design. The optimum conditions were determined as follows: papain concentration 425 U / mL, enzymatic hydrolysis temperature 62 鈩，

本文編號：1943363