本文選題：微波合成法 + 碳量子點��； 參考：《鄭州大學(xué)》2014年碩士論文

【摘要】：碳量子點作為新興的熒光納米材料，以其優(yōu)良的光學(xué)性能和低毒性逐步發(fā)展為一種新型的熒光探針。目前，碳量子點引起各國研究者的關(guān)注，已被廣泛應(yīng)用于環(huán)境檢測、活體成像、生物探針以及光電器件等諸多領(lǐng)域。碳量子點的合成方法已日趨成熟，但對碳量子點的熒光性能的影響因素研究報道較少。其中，利用量子點的特性作為熒光探針分析藥物小分子、核酸、蛋白等生物大分子含量的報道已有一些，但是利用碳量子點作為熒光探針來進行藥物分析的文獻卻并不多見。本論文試圖對碳量子點的熒光性質(zhì)影響因素進行探究；尋找新的熒光探針，建立靈敏便捷的藥物小分子定量檢測方法。以熒光猝滅法為主要的研究方法，利用紫外-可見吸收光譜、氯化鈉離子分析實驗以及熒光猝滅程度與溫度的關(guān)系等實驗方法對碳量子點與藥物的結(jié)合機理進行了探討。全文主要內(nèi)容包括如下幾個部分： 1.實驗采用簡單快速的微波加熱法合成了水溶性碳量子點，并利用透射電鏡，紫外可見吸收光譜，熒光光譜，紅外光譜及納米粒度儀對其形態(tài)學(xué)，光學(xué)及表面化學(xué)性質(zhì)進行了表征；對各種影響因素如表面活性劑、氧化劑、還原劑、常見離子、熒光染料、pH、溫度及碳納米材料等對碳量子點的熒光性質(zhì)的影響，進行初步研究和分析。此外，初步探究了四環(huán)素類和黃酮類部分藥物與碳量子點的相互作用，為該類藥物的分析提供一種檢測方法。 2.基于姜黃素對微波加熱法合成的水溶性碳量子點的熒光猝滅作用，將碳量子點作為熒光探針。研究發(fā)現(xiàn)姜黃素的加入能夠使碳量子點的熒光信號顯著降低。在pH4.0的Britton-Robinson的緩沖溶液中，姜黃素的濃度與碳量子點的熒光強度變化成線性關(guān)系，由此建立了一種姜黃素的定量方法。其中姜黃素的線性范圍在0.03-3.0μg mL-1，相關(guān)系數(shù)r=0.9984，檢測限為4.42×10-10g mL-1，將此法用于合成樣品及實際樣品分析，結(jié)果令人滿意。利用紫外可見吸收光譜，氯化鈉離子實驗和熒光猝滅程度與溫度的關(guān)系對姜黃素與碳量子點相互作用的可能機理進行了探討。 3.探究了鹽酸四環(huán)素（TC）對水溶性碳量子點的熒光猝滅作用。實驗采用碳量子點作為熒光探針，研究發(fā)現(xiàn)鹽酸四環(huán)素能夠使碳量子點的熒光信號降低。在pH4.0的Britton-Robinson的緩沖溶液中，鹽酸四環(huán)素的濃度與碳量子點的熒光強度變化成線性關(guān)系，由此建立了鹽酸四環(huán)素的定量方法。在優(yōu)化的實驗條件下，，鹽酸四環(huán)素的線性范圍在0.3-30μg mL-1，相關(guān)系數(shù)r=0.9991，檢測限為2.83×10-9g mL-1，并將此法用于合成樣品及實際樣品分析，結(jié)果令人滿意。利用紫外可見吸收光譜，氯化鈉離子實驗和熒光猝滅程度與溫度的關(guān)系對鹽酸四環(huán)素與碳量子點相互作用的可能機理進行了探討。
[Abstract]:Carbon quantum dots (QDs), as new fluorescent nanomaterials, have been developed as novel fluorescent probes with their excellent optical properties and low toxicity. At present, carbon quantum dots (QDs) have attracted the attention of researchers all over the world, and have been widely used in many fields, such as environmental detection, living imaging, biological probes and optoelectronic devices. The synthesis methods of carbon quantum dots have become more and more mature, but there are few reports on the factors influencing the fluorescence properties of carbon quantum dots. Among them, there have been some reports on the use of quantum dots as fluorescence probes for the analysis of drug micromolecules, nucleic acids, proteins and other biological macromolecules, but there are few literatures on the use of carbon quantum dots as fluorescent probes for drug analysis. In this paper, we try to explore the factors affecting the fluorescence properties of carbon quantum dots, find new fluorescent probes, and establish a sensitive and convenient method for quantitative detection of small molecules of drugs. The binding mechanism of carbon quantum dots (QDs) with drugs was investigated by UV-Vis absorption spectra, sodium chloride ion analysis and the relationship between fluorescence quenching degree and temperature. The main contents of this paper are as follows: 1. The water-soluble carbon quantum dots were synthesized by a simple and rapid microwave heating method. The morphology of the QDs was characterized by transmission electron microscope, UV-Vis absorption spectrum, fluorescence spectrum, infrared spectrum and nano-particle size analyzer. The optical and surface chemical properties were characterized, and the effects of various factors, such as surfactant, oxidant, reductant, common ions, pH of fluorescent dye, temperature and carbon nanomaterials, on the fluorescence properties of carbon quantum dots were studied. To carry out preliminary research and analysis. In addition, the interaction of tetracycline and flavonoids with carbon quantum dots (QDs) was preliminarily investigated, which provided a method for the analysis of tetracycline and flavonoids. 2. Based on the fluorescence quenching effect of curcumin on water-soluble carbon quantum dots synthesized by microwave heating, carbon quantum dots were used as fluorescence probes. It is found that curcumin can significantly reduce the fluorescence signal of carbon quantum dots. In the buffer solution of Britton-Robinson of pH4.0, the concentration of curcumin was linearly correlated with the fluorescence intensity of carbon quantum dots, and a quantitative method of curcumin was established. The linear range of curcumin was 0.03-3.0 渭 g mL ~ (-1), the correlation coefficient was 0.9984, and the detection limit was 4.42 脳 10 ~ (-10) g mL ~ (-1). The method was applied to the analysis of synthetic and actual samples with satisfactory results. The possible mechanism of the interaction between curcumin and carbon quantum dots was investigated by UV-Vis absorption spectra, sodium chloride ion experiments and the relationship between fluorescence quenching degree and temperature. 3. The fluorescence quenching effect of tetracycline hydrochloride (TC) on water soluble carbon quantum dots (QDs) was investigated. Using carbon quantum dots as fluorescence probe, it is found that tetracycline hydrochloride can reduce the fluorescence signal of carbon quantum dots. In the buffer solution of Britton-Robinson of pH4.0, the concentration of tetracycline hydrochloride was linearly correlated with the fluorescence intensity of carbon quantum dots. A quantitative method for tetracycline hydrochloride was established. Under the optimized experimental conditions, the linear range of tetracycline hydrochloride was 0.3-30 渭 g mL ~ (-1), the correlation coefficient was 0.9991, and the detection limit was 2.83 脳 10 ~ (-9) g mL ~ (-1). The method was applied to the analysis of synthetic and practical samples with satisfactory results. The possible mechanism of the interaction between tetracycline hydrochloride and carbon quantum dots was investigated by UV-Vis absorption spectra, sodium chloride ion experiments and the relationship between fluorescence quenching degree and temperature.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R917

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