本文選題：人參皂苷CK + Caco-細(xì)胞模型　； 參考：《中國(guó)藥學(xué)雜志》2015年22期

【摘要】：目的考察聚乙二醇琥珀酸酯1000(TPGS 1000)與Soluplus對(duì)人參皂苷CK在Caco-2細(xì)胞模型上轉(zhuǎn)運(yùn)的影響。方法采用Caco-2細(xì)胞模型研究不同濃度的聚乙二醇琥珀酸酯1000與Soluplus對(duì)人參皂苷CK細(xì)胞轉(zhuǎn)運(yùn)行為的影響,以超高壓液相色譜法(UPLC)測(cè)定細(xì)胞樣品溶液中人參皂苷CK的濃度,計(jì)算表觀滲透系數(shù)(Papp)與外排比率。結(jié)果當(dāng)人參皂苷CK與聚乙二醇琥珀酸酯1000或Soluplus質(zhì)量比例分別為1∶1、1∶3和1∶9時(shí),人參皂苷CK吸收顯著增加,外排顯著降低,外排比率也隨之顯著下降(P0.05);在相同比例條件下,聚乙二醇琥珀酸酯1000對(duì)人參皂苷CK的促吸收效果和抑制外排效果均優(yōu)于Soluplus(P0.05)。結(jié)論在Caco-2細(xì)胞模型上,聚乙二醇琥珀酸酯1000與Soluplus均可顯著促進(jìn)人參皂苷CK的吸收,有希望應(yīng)用于CK制劑的開發(fā)中。
[Abstract]:Aim to investigate the effects of polyethylene glycol succinate (1000(TPGS 1000) and Soluplus on the transport of ginsenoside CK on Caco-2 cell model. Methods Caco-2 cell model was used to study the effects of different concentrations of polyethylene glycol succinate 1000 and Soluplus on the transport behavior of ginsenoside CK cells. The concentration of ginsenoside CK in cell sample solution was determined by ultrahigh pressure liquid chromatography (HPLC). The apparent permeability coefficient (Pappa) and efflux ratio were calculated. Results when the mass ratio of ginsenoside CK to polyethylene glycol succinate 1000 or Soluplus was 1: 1: 1: 3 and 1:9, the absorption of ginsenoside CK was significantly increased, and the efflux of ginsenoside CK was significantly decreased. The efflux ratio also decreased significantly (P0.05A), and at the same ratio, the effects of polyethylene glycol succinate 1000 on the absorption and inhibition of ginsenoside CK were better than that of Soluplusp 0.05%. Conclusion on Caco-2 cell model, both polyethylene glycol succinate 1000 and Soluplus can significantly promote the absorption of ginsenoside CK.
【作者單位】： 江蘇省中醫(yī)藥研究院國(guó)家中醫(yī)藥管理局中藥釋藥系統(tǒng)重點(diǎn)研究室;宿遷市第一人民醫(yī)院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81403119)
【分類號(hào)】：R969.2
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本文編號(hào)：1911663